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Invitrogen
ULBP1 Recombinant Rabbit Monoclonal Antibody (4H27L8)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
ULBP1 Antibody (703182) in ICC/IF
For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous ULBP1 using Anti-ULBP1 Recombinant Rabbit Monoclonal Antibody (Product # 703182, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ULBP1 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Produc... View More
Product Details
703182
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H27L8
Immunogen
Protein corresponding to human ULBP1 [aa29-aa126]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784567
Product Specific Information
This antibody is predicted to react with Monkey, Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ULBP1 is the ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALCAN-beta; N2DL-1; NKG2D ligand; NKG2D ligand 1; NKG2D ligand1; Retinoic acid early transcript 1I; UL16-binding protein 1; ULBP; ULBP 1
Gene Aliases: N2DL-1; N2DL1; NKG2DL1; RAET1I; ULBP1
UniProt ID: (Human) Q9BZM6
Entrez Gene ID: (Human) 80329
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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