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Product Details
OSU00013W-150UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from human ULK1 (phospho S450) conjugated to an immunogenic carrier protein has been used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: ULK1. This antibody can recognize both the phosphorylated and unphosphorylated target.
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: ATG1; ATG1A; hATG1; KIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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