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Invitrogen
Ubiquilin 1 Polyclonal Antibody
FIGURE: 1 / 3
Ubiquilin 1 Antibody (PA5-22174) in WB
Western blot analysis was performed on whole cell extract (30 µg lysate) of SH-SY5Y (Lane 1), U-87 mg (Lane 2), RAW 264.7 (Lane 3), A431 (Lane 4), HeLa (Lane 5), PC-3 (Lane 6), A549 (Lane 7) and tissue extract of Mouse Brain (Lane 8). The blot was probed with Anti-Ubiquilin 1 Polyclonal Antibody (Product # PA5-22174, 1:3,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 63 kDa band corresponding to Ubiqu... View More
Product Details
PA5-22174
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 64 and 345 (Q9UMX0) of Ubiquilin 1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11157427
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (90%), Rat (90%), Sheep (96%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PLIC1 is an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hPLIC-1; PLIC-1; Protein linking IAP with cytoskeleton 1; testicular tissue protein Li 219; Ubiquilin-1
Gene Aliases: 1110046H03Rik; 1810030E05Rik; AU019746; C77538; D13Ertd372e; DA41; DSK2; PLIC-1; PLIC1; UBQLN1; UBQN; XDRP1
UniProt ID: (Human) Q9UMX0, (Mouse) Q8R317
Entrez Gene ID: (Human) 29979, (Mouse) 56085
autophagosome assembly
macroautophagy
ER-associated ubiquitin-dependent protein catabolic process
regulation of protein ubiquitination
positive regulation of protein ubiquitination
negative regulation of toll-like receptor 3 signaling pathway
response to endoplasmic reticulum stress
aggrephagy
cellular response to hypoxia
autophagosome maturation
negative regulation of store-operated calcium channel activity
regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of ER-associated ubiquitin-dependent protein catabolic process
regulation of macroautophagy
negative regulation of autophagosome maturation
regulation of autophagosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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