Invitrogen

Ubiquitin Recombinant Rabbit Monoclonal Antibody (10H4L21)

4 Published Figures
8 References
View all (60) Ubiquitin antibodies
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Datasheet
Protocols
Questions & Answers
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Product Details

701339

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1 µg/mL
View 2 publications 2 publications

ChIP assay (ChIP)

1 µL
-

in situ PLA (PLA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

10H4L21

Immunogen

Peptide corresponding to amino acids 32-58 of human ubiquitin
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532471

Product Specific Information

701339 was successfully used to detect the ubiquitination of GLUT1 in transfected in HeLa cells.

This antibody is predicted to react with bovine, chimpanzee, equine, non-human primate, rabbit, ovine and porcine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27a; CEP52; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27-like; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin-52 amino acid fusion protein; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein

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Gene Aliases: CEP52; CEP80; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80

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UniProt ID: (Human) P62979, (Human) Q71UM5, (Human) P62987, (Human) P0CG47, (Human) P0CG48

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Entrez Gene ID: (Human) 6233, (Human) 51065, (Human) 7311, (Human) 7314, (Human) 7316

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Function(s)
structural constituent of ribosome protein binding poly(A) RNA binding metal ion binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding ribosomal protein translational protein
Process(es)
G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator ribosomal small subunit assembly DNA repair activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation positive regulation of defense response to virus by host cellular protein modification process xenophagy positive regulation of protein ubiquitination mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential regulation of proteasomal protein catabolic process regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination ubiquitin homeostasis
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