Invitrogen

Ubiquitin Monoclonal Antibody (6C11-B3), FITC

View all (60) Ubiquitin antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

MA5-45231

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Rat, Bovine, Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

6C11-B3

Immunogen

Native bovine ubiquitin, conjugated to KLH
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931685

Product Specific Information

1 µg/mL of MA5-45231 was sufficient for detection of ubiquitin in 10 µg of Hela Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 10kDa.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; polyubiquitin; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27; ribosomal protein S27-like; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein

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Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80

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UniProt ID: (Rat) P62982, (Bovine) P62992, (Human) P62979, (Bovine) Q3T0B7, (Human) Q71UM5, (Mouse) P62983, (Rat) P24051, (Mouse) Q6ZWY3, (Human) P62987, (Bovine) P63048, (Bovine) P0CG53, (Human) P0CG47, (Human) P0CG48, (Bovine) P0CH28, (Rat) P62986, (Mouse) P62984, (Rat) P0CG51, (Mouse) P0CG49, (Mouse) Q29120, (Rat) Q63429

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Entrez Gene ID: (Rat) 100912032, (Bovine) 286839, (Human) 6233, (Bovine) 614220, (Human) 51065, (Mouse) 78294, (Rat) 681429, (Mouse) 67941, (Human) 7311, (Bovine) 615199, (Bovine) 281370, (Human) 7314, (Human) 7316, (Bovine) 444874, (Rat) 64156, (Mouse) 22186, (Rat) 192255, (Mouse) 22187, (Mouse) 22190, (Rat) 50522

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Function(s)
structural constituent of ribosome metal ion binding protein binding poly(A) RNA binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding ribosomal protein translational protein
Process(es)
translation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translational initiation SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation ribosomal small subunit assembly DNA repair cellular response to DNA damage stimulus biological_process positive regulation of defense response to virus by host cellular protein modification process xenophagy male meiosis I female meiosis I female gonad development hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination positive regulation of protein ubiquitination ubiquitin homeostasis male gonad development adipose tissue development ubiquitin-dependent protein catabolic process
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