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Invitrogen
H2AK119ub Polyclonal Antibody
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
H2AK119ub Antibody (720148) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HeLa cells for detection of H2A-Ub using H2A-Ub Rabbit Polyclonal Antibody (Product # 720148, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of H2A-Ub protein (gre... View More
Product Details
720148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to Human HIST1H2AA (aa 116-124)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605927
Product Specific Information
This antibody is predicted to react with Monkey, Pig and Cat.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H2A histone family, member R; H2A-clustered histone 1; H2ALys119ub; histone 1, H2aa; Histone H2A type 1-A; Histone H2A/r; Ubiquityl-Histone 2A K119; Ubiquityl-Histone 2A Lys119
Gene Aliases: bA317E16.2; H2AA; H2AC1; H2AFR; HIST1H2AA; TH2A
UniProt ID: (Human) Q96QV6
Entrez Gene ID: (Human) 221613
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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