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Urokinase Antibody (PA5-34790) in ICC/IF
Immunofluorescence analysis of Urokinase was performed using 70% confluent log phase PC-3 and LNCaP, control and PTI treated, cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Urokinase Polyclonal Antibody (Product # PA5-34790) at 1:100 dilution in ... View More
Product Details
PA5-34790
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 177 and 425 of Urokinase (Uniprot ID#P00749)
Conjugate
Form
Liquid
Concentration
0.77 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552142
Product Specific Information
Recommended positive controls: IMR32.
Predicted reactivity: Dog (81%), Pig (82%), Rabbit (85%), Sheep (81%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antagonist of uPA; HGNC:9052; plasminogen activator; plasminogen activator, urinary; U-plasminogen activator; UPAR; URKR; Urokinase-type plasminogen activator
Gene Aliases: ATF; BDPLT5; PLAU; QPD; u-PA; UPA; URK
UniProt ID: (Human) P00749
Entrez Gene ID: (Human) 5328
angiogenesis
proteolysis
chemotaxis
signal transduction
spermatogenesis
embryo implantation
blood coagulation
positive regulation of cell proliferation
regulation of receptor activity
response to activity
smooth muscle cell migration
regulation of smooth muscle cell migration
positive regulation of smooth muscle cell migration
positive regulation of cell migration
regulation of cell adhesion mediated by integrin
cellular response to hepatocyte growth factor stimulus
fibrinolysis
skeletal muscle tissue regeneration
response to hyperoxia
positive regulation of ovulation
regulation of wound healing
neuron death
cellular response to lipopolysaccharide
cellular response to glucose stimulus
cellular response to hypoxia
cellular response to fluid shear stress
cellular response to staurosporine
regulation of smooth muscle cell-matrix adhesion
regulation of hepatocyte proliferation
positive regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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