Invitrogen

V5 Tag Monoclonal Antibody, AP

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V5 Tag Antibody (R962-25) in WB

Western blot analysis of V5 tag was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 cells transiently overexpressing V5-His- LacZ using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. V5-His- LacZ was detected at ~117 kDa using V5-AP Mouse Monoclonal Antibody (Product # R962... View More

V5 Tag Antibody in Immunoprecipitation (IP)

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Product Details

R962-25

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

Conjugate

AP AP AP

Form

Liquid

Concentration

1-2 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556566

Product Specific Information

NOTE: R962-25 was previously sold under product #46-0287. When re-ordering, please use #R962-25.

R962-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk.

In Western blot applications, no cross-reactivity has been observed in bacterial lysates.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Humans with inherited T cell CD28 deficiency are susceptible to skin papillomaviruses but are otherwise healthy.

Cell

Béziat V,Rapaport F,Hu J,Titeux M,Bonnet des Claustres M,Bourgey M,Griffin H,Bandet É,Ma CS,Sherkat R,Rokni-Zadeh H,Louis DM,Changi-Ashtiani M,Delmonte OM,Fukushima T,Habib T,Guennoun A,Khan T,Bender N,Rahman M,About F,Yang R,Rao G,Rouzaud C,Li J,Shearer D,Balogh K,Al Ali F,Ata M,Dabiri S,Momenilandi M,Nammour J,Alyanakian MA,Leruez-Ville M,Guenat D,Materna M,Marcot L,Vladikine N,Soret C,Vahidnezhad H,Youssefian L,Saeidian AH,Uitto J,Catherinot É,Navabi SS,Zarhrate M,Woodley DT,Jeljeli M,Abraham T,Belkaya S,Lorenzo L,Rosain J,Bayat M,Lanternier F,Lortholary O,Zakavi F,Gros P,Orth G,Abel L,Prétet JL,Fraitag S,Jouanguy E,Davis MM,Tangye SG,Notarangelo LD,Marr N,Waterboer T,Langlais D,Doorbar J,Hovnanian A,Christensen N,Bossuyt X,Shahrooei M,Casanova JL

R962-25 was used in Western Blotting to conclude that human CD28-dependent T cell responses are largely redundant for protective immunity.

Thu Jul 08 00:00:00 EDT 2021

DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.

The EMBO journal

Ben Yamin B,Ahmed-Seghir S,Tomida J,Despras E,Pouvelle C,Yurchenko A,Goulas J,Corre R,Delacour Q,Droin N,Dessen P,Goidin D,Lange SS,Bhetawal S,Mitjavila-Garcia MT,Baldacci G,Nikolaev S,Cadoret JC,Wood RD,Kannouche PL

Published figure using V5 Tag monoclonal antibody (Product # R962-25) in Immunoprecipitation

Tue Nov 02 00:00:00 EDT 2021

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

The Journal of experimental medicine

Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,Müller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,Béziat V,Casanova JL

R96225 was used in western blot to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells

Mon Oct 17 00:00:00 EDT 2016

Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).

The Journal of biological chemistry

Takata K,Tomida J,Reh S,Swanhart LM,Takata M,Hukriede NA,Wood RD

R962-25 was used in immunoprecipitation to study the expression, biological roles, and biochemical activities of DNA polymerase v

Fri Oct 02 00:00:00 EDT 2015

Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells.

Cancer letters

Silveira AC,Hurst DR,Vaidya KS,Ayer DE,Welch DR

R962-25 was used in Western Blotting to demonstrate functional differences in BRMS1 family members, and highlight the differences in SIN3-HDAC composition affecting protein expression and biological behaviours.

Wed Apr 08 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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