Invitrogen

V5 Tag Polyclonal Antibody

FIGURE: 1 / 8

V5 Tag Antibody (PA1-993) in WB

Western blot was performed using Anti-V5 Tag Polyclonal Antibody (Product # PA1-993) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), V5-H3-His, 50 µg (Lane 3), Myc-p65-V5, 50 µg (Lane 4), Myc-p65-V5, 25 µg (Lane 5), Myc-p65-V5, 12.5 µg (Lane 6), Myc-p65-V5, 6.26 µg (Lane 7), p65-HA, 50 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved protei... View More

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Product Details

PA1-993

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues G K P I P N P L L G L D S T.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_561893

Product Specific Information

PA1-993 detects V5 tagged proteins.

PA1-993 has been used successfully in Western blot procedures. By Western blot this antibody detects the presence of V5-tagged proteins.

The PA1-993 immunogen corresponds to the V5 tag.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The SUMO ligase MMS21 profoundly influences maize development through its impact on genome activity and stability.

PLoS genetics

Zhang J,Augustine RC,Suzuki M,Feng J,Char SN,Yang B,McCarty DR,Vierstra RD

Published figure using V5 Tag polyclonal antibody (Product # PA1-993) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Functional α6β4 acetylcholine receptor expression enables pharmacological testing of nicotinic agonists with analgesic properties.

The Journal of clinical investigation

Knowland D,Gu S,Eckert WA,Dawe GB,Matta JA,Limberis J,Wickenden AD,Bhattacharya A,Bredt DS

PA1-993 was used in Western Blotting to identify roles for IRE1α and BARP in neurotransmitter receptor assembly and unlock drug discovery for the previously elusive α6β4 receptor.

Mon Nov 02 00:00:00 EST 2020

Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells.

Nucleus (Austin, Tex.)

Cheng LC,Baboo S,Lindsay C,Brusman L,Martinez-Bartolomé S,Tapia O,Zhang X,Yates JR,Gerace L

PA1-993 was used in Immunocytochemistry-immunoflourescence to provide new focal points for studying the functions of the NE.

Sun Dec 01 00:00:00 EST 2019

Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas.

The Plant cell

Kong F,Burlacot A,Liang Y,Légeret B,Alseekh S,Brotman Y,Fernie AR,Krieger-Liszkay A,Beisson F,Peltier G,Li-Beisson Y

PA1-993 was used in Western Blotting to conclude that peroxisomal MDH2 helps photoautotrophs cope with nitrogen scarcity and high light by transmitting the redox state of the peroxisome to the chloroplast by means of malate shuttle- and H2O2-based redox signaling.

Wed Aug 01 00:00:00 EDT 2018

RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells.

American journal of physiology. Cell physiology

Gallo LI,Dalghi MG,Clayton DR,Ruiz WG,Khandelwal P,Apodaca G

Published figure using V5 Tag polyclonal antibody (Product # PA1-993) in Western Blot

Thu Mar 01 00:00:00 EST 2018

S100A4 as a Target of the E3-Ligase Asb2β and Its Effect on Engineered Heart Tissue.

Frontiers in physiology

Braumann S,Thottakara T,Stücker S,Reischmann-Düsener S,Krämer E,Groß J,Hirt MN,Doroudgar S,Carrier L,Friedrich FW

PA1-993 was used in Western Blotting to analyse S100A4 expression in models of cardiac pathology, investigate its degradation by the ubiquitin-proteasome system, and examine the functional effects of S100A4 levels in a 3D model of engineered heart tissue.

Thu Oct 01 00:00:00 EDT 2020

An N-terminal motif unique to primate tau enables differential protein-protein interactions.

The Journal of biological chemistry

Stefanoska K,Volkerling A,Bertz J,Poljak A,Ke YD,Ittner LM,Ittner A

PA1-993 was used in Immunoprecipitation to study the function of an 11-amino acid-long motif in the N-terminal region of primate Tau.

Fri Mar 09 00:00:00 EST 2018

Mutations in TGM6 induce the unfolded protein response in SCA35.

Human molecular genetics

Tripathy D,Vignoli B,Ramesh N,Polanco MJ,Coutelier M,Stephen CD,Canossa M,Monin ML,Aeschlimann P,Turberville S,Aeschlimann D,Schmahmann JD,Hadjivassiliou M,Durr A,Pandey UB,Pennuto M,Basso M

PA1-993 was used in Immunocytochemistry-immunoflourescence to study the molecular basis of mutant transglutaminase 6-induced neurotoxicity, linked to spinocerebellar ataxia type 35.

Sun Oct 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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