Invitrogen

V5 Tag Monoclonal Antibody (2F11F7), Alexa Fluor™ 488

V5 Tag Antibody in Immunocytochemistry (ICC/IF)

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V5 Tag Antibody (37-7500-A488) in ICC/IF

Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5-H3-6XHis transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Monoclonal Antibody (2F11F7), Alexa Fluor 488 (Product # 37-7500-A488) at 5 µg/mL and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then label... View More

V5 Tag Antibody in Immunoprecipitation (IP)

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Product Details

37-7500-A488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F11F7

Immunogen

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610630

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity.

Science advances

Grzejda D,Mach J,Schweizer JA,Hummel B,Rezansoff AM,Eggenhofer F,Panhale A,Lalioti ME,Cabezas Wallscheid N,Backofen R,Felsenberg J,Hilgers V

37-7500-A488 was used in Immunohistochemistry to report an architectural RNA for a neuronal granule and provides a handle to interrogate functions of a condensate independently of those of its constituent proteins.

Fri Sep 30 00:00:00 EDT 2022

Development of an HIV reporter virus that identifies latently infected CD4⁺ T cells.

Cell reports methods

Kim EH,Manganaro L,Schotsaert M,Brown BD,Mulder LCF,Simon V

37-7500-A488 was used in Flow cytometry/Cell sorting to show that this viral vector permits the interrogation of HIV latency at single-cell resolution, revealing mechanisms of latency establishment and allowing the characterization of effective latency-reversing agents.

Mon Jun 20 00:00:00 EDT 2022

APEX Proximity Labeling of Stress Granule Proteins.

Methods in molecular biology (Clifton, N.J.)

Elmsaouri S,Markmiller S,Yeo GW

37-7500-A488 was used in Western Blotting to describe the utility of APEX-mediated proximity labeling to recover components of stress granules (SGs) by endogenous tagging of well-established SG-associated proteins.

Fri Feb 18 00:00:00 EST 2022

Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.

Nature

Braga L,Ali H,Secco I,Chiavacci E,Neves G,Goldhill D,Penn R,Jimenez-Guardeño JM,Ortega-Prieto AM,Bussani R,Cannatà A,Rizzari G,Collesi C,Schneider E,Arosio D,Shah AM,Barclay WS,Malim MH,Burrone J,Giacca M

37-7500-A488 was used in Immunocytochemistry to suggest a potential mechanism for COVID-19 disease pathogenesis and support the repurposing of niclosamide for therapy.

Tue Jun 01 00:00:00 EDT 2021

SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care.

Nature communications

Gutmann C,Takov K,Burnap SA,Singh B,Ali H,Theofilatos K,Reed E,Hasman M,Nabeebaccus A,Fish M,McPhail MJ,O'Gallagher K,Schmidt LE,Cassel C,Rienks M,Yin X,Auzinger G,Napoli S,Mujib SF,Trovato F,Sanderson B,Merrick B,Niazi U,Saqi M,Dimitrakopoulou K,Fernández-Leiro R,Braun S,Kronstein-Wiedemann R,Doores KJ,Edgeworth JD,Shah AM,Bornstein SR,Tonn T,Hayday AC,Giacca M,Shankar-Hari M,Mayr M

37-7500-A488 was used in Immunocytochemistry to show that proteins of the complement system and galectin-3-binding protein (LGALS3BP) are identified as interaction partners of SARS-CoV-2 spike glycoprotein.

Mon Jun 07 00:00:00 EDT 2021

Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria.

eLife

Chojnowski A,Ong PF,Wong ES,Lim JS,Mutalif RA,Navasankari R,Dutta B,Yang H,Liow YY,Sze SK,Boudier T,Wright GD,Colman A,Burke B,Stewart CL,Dreesen O

Published figure using V5 Tag monoclonal antibody (Product # 37-7500-A488) in Immunoprecipitation

Thu Aug 27 00:00:00 EDT 2015

Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria.

eLife

Chojnowski A,Ong PF,Wong ES,Lim JS,Mutalif RA,Navasankari R,Dutta B,Yang H,Liow YY,Sze SK,Boudier T,Wright GD,Colman A,Burke B,Stewart CL,Dreesen O

Published figure using V5 Tag monoclonal antibody (Product # 37-7500-A488) in Immunoprecipitation

Thu Aug 27 00:00:00 EDT 2015

Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria.

eLife

Chojnowski A,Ong PF,Wong ES,Lim JS,Mutalif RA,Navasankari R,Dutta B,Yang H,Liow YY,Sze SK,Boudier T,Wright GD,Colman A,Burke B,Stewart CL,Dreesen O

Published figure using V5 Tag monoclonal antibody (Product # 37-7500-A488) in Immunoprecipitation

Thu Aug 27 00:00:00 EDT 2015

Genetic alterations in the phosphoinositide 3-kinase/Akt signaling pathway confer sensitivity of thyroid cancer cells to therapeutic targeting of Akt and mammalian target of rapamycin.

Cancer research

Liu D,Hou P,Liu Z,Wu G,Xing M

Published figure using V5 Tag monoclonal antibody (Product # 37-7500-A488) in Western Blot

Tue Sep 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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V5 Tag Monoclonal Antibody (2F11F7), Alexa Fluor™ 488

V5 Tag Antibody (37-7500-A488) in ICC/IF

Product Details

37-7500-A488

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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