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Invitrogen
V5 Tag Monoclonal Antibody (TCM5), PE-Cyanine7, eBioscience™
FIGURE: 1 / 1
V5 Tag Antibody (25-6796-42) in Flow
293T cells transfected with a construct co-expressing a GFP reporter gene and a V5-tagged surface protein were stained with mouse IgG2b kappa Isotype Control, PE-Cyanine7 (Product # 25-4732-81) (left) or V5 tag Monoclonal Antibody, PE-Cyanine7 (Product # 25-6796-42) (right). Total viable cells were used for analysis.
Product Details
25-6796-42
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TCM5
Immunogen
Peptide sequence-GKPIPNPLLGLDST
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784669
Product Specific Information
Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.
Applications Reported: This TCM5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TCM5 antibody has been pre-diluted and tested by flow cytometric analysis of the human 293T cancer cell line transfected with the V5 tagged Type II transmembrane protein CD207 and a GFP reporter gene. This may be used at 5 µL (0.06 µg) per test for surface staining and at 1.25 µL (0.015 µg) per test for intracellular staining. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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