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VAChT Antibody (600-401-MK7) in ICC/IF
Immunofluorescence of Rabbit Anti-VAChT Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Primary Antibody: Rabbit Anti-VAChT Antibody at 15µg/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated (p/n 611-741-127) at 5µg/mL for 1hr at RT. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Nuclear Counter Stain: DAPI. Image: A) DAPI (blue). B) Anti-VAChT (green) and Secondary. C) Merge of A+B images. D) Secondary only. Localization: predicted membrane, Golgi apparatus... View More
Product Details
600-401-MK7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-VAChT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of rat vesicular acetylcholine transporter conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against rat VAChT. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and human.
Target Information
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
Gene Aliases: rVAT; SLC18A3; VACHT; Vat
UniProt ID: (Human) B2R7S1, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Rat) 60422
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