Proteintech

VASP Polyclonal Antibody

VASP Antibody in Immunocytochemistry (ICC/IF)

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VASP Antibody (13472-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using VASP antibody 13472-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green).

VASP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VASP Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13472-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Mouse, Rat, Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VASP Fusion Protein Ag4266 (1-380 aa encoded by BC038224)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to VASP.

Immunogen sequence: MSETVICSS RATVMLYDDG NKRWLPAGTG PQAFSRVQIY HNPTANSFRV VGRKMQPDQQ VVINCAIVRG VKYNQATPNF HQWRDARQVW GLNFGSKEDA AQFAAGMASA LEALEGGGPP PPPALPTWSV PNGPSPEEVE QQKRQQPGPS EHIERRVSNA GGPPAPPAGG PPPPPGPPPP PGPPPPPGLP PSGVPAAAHG AGGGPPPAPP LPAAQGPGGG GAGAPGLAAA IAGAKLRKVS KQEEASGGPT APKAESGRSG GGGLMEEMNA MLARRRKATQ VGEKTPKDES ANQEEPEARV PAQSESVRRP WEKNSTTLPR MKSSSSVTTS ETQPCTPSSS DYSDLQRVKQ ELLEEVKKEL QKVKEEIIEA FVQELRKRGS P (1-380 aa encoded by BC038224)

Target Information

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative Proteomic Analysis Reveals apoE4-Dependent Phosphorylation of the Actin-Regulating Protein VASP.

Molecular & cellular proteomics : MCP

Cakir Z,Lord SJ,Zhou Y,Jang GM,Polacco BJ,Eckhardt M,Jimenez-Morales D,Newton BW,Orr AL,Johnson JR,da Cruz A,Mullins RD,Krogan NJ,Mahley RW,Swaney DL

Mon May 01 00:00:00 EDT 2023

Quantitative Proteomic Analysis Reveals apoE4-Dependent Phosphorylation of the Actin-Regulating Protein VASP.

Molecular & cellular proteomics : MCP

Cakir Z,Lord SJ,Zhou Y,Jang GM,Polacco BJ,Eckhardt M,Jimenez-Morales D,Newton BW,Orr AL,Johnson JR,da Cruz A,Mullins RD,Krogan NJ,Mahley RW,Swaney DL

Mon May 01 00:00:00 EDT 2023

Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.

Experimental and therapeutic medicine

Hu H,Li C,Zhang H,Wu G,Huang Y

Sat May 01 00:00:00 EDT 2021

Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.

Experimental and therapeutic medicine

Hu H,Li C,Zhang H,Wu G,Huang Y

Sat May 01 00:00:00 EDT 2021

The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.

Cell death & disease

Xiang T,Yuan C,Guo X,Wang H,Cai Q,Xiang Y,Luo W,Liu G

Thu Feb 04 00:00:00 EST 2021

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

Frontiers in oncology

Wang J,Liu G,Liu M,Cai Q,Yao C,Chen H,Song N,Yuan C,Tan D,Hu Y,Xiang Y,Xiang T

Wed Nov 08 00:00:00 EST 2023

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Oncogene

Li K,Zhang J,Tian Y,He Y,Xu X,Pan W,Gao Y,Chen F,Wei L

Sun Mar 01 00:00:00 EST 2020

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Oncogene

Li K,Zhang J,Tian Y,He Y,Xu X,Pan W,Gao Y,Chen F,Wei L

Sun Mar 01 00:00:00 EST 2020

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Oncogene

Li K,Zhang J,Tian Y,He Y,Xu X,Pan W,Gao Y,Chen F,Wei L

Sun Mar 01 00:00:00 EST 2020

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

PLoS biology

Lv Y,Wang X,Li X,Xu G,Bai Y,Wu J,Piao Y,Shi Y,Xiang R,Wang L

Sun Nov 01 00:00:00 EDT 2020

Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis.

Pathology, research and practice

Li K,Ma YB,Tian YH,Xu XL,Gao Y,He YQ,Pan WT,Zhang JW,He CJ,Wei L

Tue Oct 01 00:00:00 EDT 2019

TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.

Oncology reports

Liu W,Chen X,He Y,Tian Y,Xu L,Ma Y,Hu P,Su K,Luo Z,Wei L,Zhang J

Mon Apr 01 00:00:00 EDT 2019

Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

Nature communications

Courtheoux T,Enchev RI,Lampert F,Gerez J,Beck J,Picotti P,Sumara I,Peter M

Mon Sep 19 00:00:00 EDT 2016

Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

Nature communications

Courtheoux T,Enchev RI,Lampert F,Gerez J,Beck J,Picotti P,Sumara I,Peter M

Mon Sep 19 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Enah/Vasp-like; Vasodilator-stimulated phosphoprotein; VASP

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Gene Aliases: VASP

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UniProt ID: (Human) P50552, (Mouse) P70460

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Entrez Gene ID: (Human) 7408, (Rat) 361517, (Mouse) 22323

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Function(s)

actin binding protein binding profilin binding SH3 domain binding cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

Process(es)

neural tube closure axon guidance actin polymerization or depolymerization positive regulation of actin filament polymerization cell junction assembly protein homotetramerization cell-cell adhesion actin cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VASP Polyclonal Antibody

VASP Antibody (13472-1-AP) in ICC/IF

Product Details

13472-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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