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VCAM-1 Antibody (500-P300-1MG) in ELISA
Sandwich ELISA: To detect Human VCAM-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of VCAM-1 Polyclonal Antibody (Product # 500-P300-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech VCAM-1 Polyclonal Antibody, Biotin (Product # 500-P300BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VCAM-1.
Product Details
500-P300-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
HEK293 cells-derived Recombinant Human VCAM-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930300
Product Specific Information
AA Sequence of recombinant protein: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VCAM-1. Anti-Human VCAM-1-specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix.
Sandwich ELISA: To detect Human VCAM-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VCAM-1 (500-P300Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VCAM-1.
Western Blot: To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1
Gene Aliases: CD106; INCAM-100; VCAM1
UniProt ID: (Human) P19320
Entrez Gene ID: (Human) 7412
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
B cell differentiation
extracellular matrix organization
response to lipopolysaccharide
response to nicotine
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
regulation of immune response
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
interferon-gamma-mediated signaling pathway
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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