Invitrogen

VCP Polyclonal Antibody

View all (37) VCP antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-120833

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 460-770 of human VCP (NP_009057.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.189 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914405

Product Specific Information

Positive test controls include: HeLa, K-562, SH-SY5Y, Mouse brain, Mouse spleen, Mouse kidney, Rat liver, Rat brain. The target is usually found in the following locations: Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol.

Immunogen sequence: NPSALRETVV EVPQVTWEDI GGLEDVKREL QELVQYPVEH PDKFLKFGMT PSKGVLFYGP PGCGKTLLAK AIANECQANF ISIKGPELLT MWFGESEANV REIFDKARQA APCVLFFDEL DSIAKARGGN IGDGGGAADR VINQILTEMD GMSTKKNVFI IGATNRPDII DPAILRPGRL DQLIYIPLPD EKSRVAILKA NLRKSPVAKD VDLEFLAKMT NGFSGADLTE ICQRACKLAI RESIESEIRR ERERQTNPSA MEVEEDDPVP EIRRDHFEEA MRFARRSVSD NDIRKYEMFA QTLQQSRGFG S

Target Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; or HEL-S-70; p97 atpase; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP; yeast Cdc48p homolog

View more View less

Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; TERA; VCP

View more View less

UniProt ID: (Human) P55072, (Mouse) Q01853, (Rat) P46462

View more View less

Entrez Gene ID: (Human) 7415, (Mouse) 269523, (Rat) 116643

View more View less

Function(s)
protein binding ATP binding lipid binding ATPase activity protein phosphatase binding protein domain specific binding polyubiquitin binding ubiquitin protein ligase binding deubiquitinase activator activity K48-linked polyubiquitin binding MHC class I protein binding identical protein binding ADP binding ubiquitin-like protein ligase binding poly(A) RNA binding BAT3 complex binding ubiquitin-specific protease binding nucleotide binding receptor binding hydrolase activity protein complex binding
Process(es)
DNA repair double-strand break repair ubiquitin-dependent protein catabolic process transport ER to Golgi vesicle-mediated transport activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus protein ubiquitination protein N-linked glycosylation via asparagine viral genome replication translesion synthesis ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol positive regulation of protein complex assembly positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein hexamerization positive regulation of protein catabolic process protein homooligomerization aggresome assembly ER-associated misfolded protein catabolic process positive regulation of protein K63-linked deubiquitination positive regulation of Lys63-specific deubiquitinase activity NADH metabolic process autophagy positive regulation of mitochondrial membrane potential endoplasmic reticulum unfolded protein response ERAD pathway regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process establishment of protein localization ATP metabolic process error-free translesion synthesis flavin adenine dinucleotide catabolic process autophagosome maturation regulation of aerobic respiration positive regulation of oxidative phosphorylation positive regulation of ubiquitin-specific protease activity positive regulation of ATP biosynthetic process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support