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Invitrogen
VCP Polyclonal Antibody
Advanced Verification
This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 16
VCP Antibody (PA5-22257) in IHC (P)
VCP Polyclonal Antibody detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. VCP Polyclonal Antibody (Product # PA5-22257) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-22257
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID#P55072)
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11157831
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (98%), Pig (99%), Chicken (99%), Chimpanzee (99%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; or HEL-S-70; p97 atpase; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP; yeast Cdc48p homolog
Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; TERA; VCP
UniProt ID: (Human) P55072, (Mouse) Q01853, (Rat) P46462
Entrez Gene ID: (Dog) 481590, (Human) 7415, (Mouse) 269523, (Rat) 116643
protein binding
ATP binding
lipid binding
ATPase activity
protein phosphatase binding
protein domain specific binding
polyubiquitin binding
ubiquitin protein ligase binding
deubiquitinase activator activity
K48-linked polyubiquitin binding
MHC class I protein binding
identical protein binding
ADP binding
ubiquitin-like protein ligase binding
poly(A) RNA binding
BAT3 complex binding
ubiquitin-specific protease binding
nucleotide binding
receptor binding
hydrolase activity
protein complex binding
DNA repair
double-strand break repair
ubiquitin-dependent protein catabolic process
transport
ER to Golgi vesicle-mediated transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
protein ubiquitination
protein N-linked glycosylation via asparagine
viral genome replication
translesion synthesis
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
positive regulation of protein complex assembly
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein hexamerization
positive regulation of protein catabolic process
protein homooligomerization
aggresome assembly
ER-associated misfolded protein catabolic process
positive regulation of protein K63-linked deubiquitination
positive regulation of Lys63-specific deubiquitinase activity
NADH metabolic process
autophagy
positive regulation of mitochondrial membrane potential
endoplasmic reticulum unfolded protein response
ERAD pathway
regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
establishment of protein localization
ATP metabolic process
error-free translesion synthesis
flavin adenine dinucleotide catabolic process
autophagosome maturation
regulation of aerobic respiration
positive regulation of oxidative phosphorylation
positive regulation of ubiquitin-specific protease activity
positive regulation of ATP biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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