Invitrogen

VCP Monoclonal Antibody (5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

46 Published Figures

42 References

View all (37) VCP antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

VCP Antibody in Immunoprecipitation (IP)

FIGURE: 1 / 63

VCP Antibody (MA3-004) in IP

Immunoprecipitation of VCP was performed on U2OS WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of VCP Monoclonal Antibody (5) (Product # MA3-004) and 30 µL of Dynabeads™ Protein G (Product # 10004D) to 500 µl of Pierce™ IP Lysis Buffer (Product # 87788). The mixture was rocked for ~1 hour at 4 degree celcius followed by two washes to remove unbound antibodies. U2OS WT cells were lysed in Pierce™ IP Lysis Buffer (Product # 87788) supplemented with protease inhibitor. The lysate was rocked for 30 min at 4 degree celcius... View More

VCP Antibody in Immunocytochemistry (ICC/IF)

VCP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VCP Antibody in Immunohistochemistry (IHC)

VCP Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

VCP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with VCP siRNA and reduction of signal was observed in Western Blot using VCP Polyclonal Antibody (Product # MA3-004). {KD}

Product Details

MA3-004

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 6 publications 6 publications

Flow Cytometry (Flow)

1/400

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2214638

Product Specific Information

MA3-004 detects VCP protein in human, mouse, and rat samples.

MA3-004 has successfully been used in Western blot immunoprecipitation, and immunohistochemical procedures. By Western blot, this antibody detects an ~97 kDa protein representing VCP from total lysate of cultured human B cells.

The MA3-004 immunogen is a synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP. This peptide (Cat. # PEP-239) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology

Iannibelli E,Gibertini S,Cheli M,Blasevich F,Cavaliere A,Riolo G,Ruggieri A,Maggi L

MA3-004 was used in Immunohistochemistry-immunofluorescence to suggest that VCP gene mutations can be related with a predominant skeletal muscle phenotype without any central nervous system involvement,.

Tue Apr 25 00:00:00 EDT 2023

VCP/p97 cofactor UBXN1/SAKS1 regulates mitophagy by modulating MFN2 removal from mitochondria.

Autophagy

Mengus C,Neutzner M,Bento ACPF,Bippes CC,Kohler C,Decembrini S,Häusel J,Hemion C,Sironi L,Frank S,Scholl HPN,Neutzner A

MA3-004 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to connect the VCP cofactor UBXN1 to the initiation and maintenance phase of PRKN-dependent mitophagy, and indicate that, upon mitochondrial stress induction, MFN2 removal from mitochondria occurs through a specialized process.

Sat Jan 01 00:00:00 EST 2022

VCP/p97 cofactor UBXN1/SAKS1 regulates mitophagy by modulating MFN2 removal from mitochondria.

Autophagy

Mengus C,Neutzner M,Bento ACPF,Bippes CC,Kohler C,Decembrini S,Häusel J,Hemion C,Sironi L,Frank S,Scholl HPN,Neutzner A

Sat Jan 01 00:00:00 EST 2022

An autopsy case of amyotrophic lateral sclerosis with striatonigral and pallidoluysian degeneration and cat's-eye-shaped neuronal nuclear inclusions.

Neuropathology : official journal of the Japanese Society of Neuropathology

Kon T,Mori F,Kinoshita I,Nakamura T,Nishijima H,Suzuki C,Goto S,Kijima H,Tomiyama M,Wakabayashi K

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunohistochemistry

Mon Aug 01 00:00:00 EDT 2022

Temporal proteomics reveal specific cell cycle oncoprotein downregulation by p97/VCP inhibition.

Cell chemical biology

Wang F,Li S,Houerbi N,Chou TF

MA3-004 was used in Western Blotting to track HCT116 colon cancer cells using temporal proteomics to define the cellular and molecular responses to proteasome and p97 inhibition.

Thu Mar 17 00:00:00 EDT 2022

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Science (New York, N.Y.)

Gwon Y,Maxwell BA,Kolaitis RM,Zhang P,Kim HJ,Taylor JP

MA3-004 was used in Western Blotting to investigate if targeting of G3BP1 weakened the stress granule-specific interaction network, resulting in granule disassembly.

Fri Jun 25 00:00:00 EDT 2021

Impacts of p97 on Proteome Changes in Human Cells during Coronaviral Replication.

Cells

Cheng KW,Li S,Wang F,Ruiz-Lopez NM,Houerbi N,Chou TF

MA3-004 was used in Western Blot to indicate a role for the essential host protein p97 in supporting HCoV replication, suggesting that p97 is a therapeutic target to treat HCoV infection.

Fri Oct 29 00:00:00 EDT 2021

Impacts of p97 on Proteome Changes in Human Cells during Coronaviral Replication.

Cells

Cheng KW,Li S,Wang F,Ruiz-Lopez NM,Houerbi N,Chou TF

MA3-004 was used in Western Blot to indicate a role for the essential host protein p97 in supporting HCoV replication, suggesting that p97 is a therapeutic target to treat HCoV infection.

Fri Oct 29 00:00:00 EDT 2021

Functional Inhibition of Valosin-Containing Protein Induces Cardiac Dilation and Dysfunction in a New Dominant-Negative Transgenic Mouse Model.

Cells

Sun X,Zhou N,Ma B,Wu W,Stoll S,Lai L,Qin G,Qiu H

MA3-004 was used in Western Blot, Immunoprecipitation to determine the effects of impaired VCP activity on the heart.

Tue Oct 26 00:00:00 EDT 2021

Functional Inhibition of Valosin-Containing Protein Induces Cardiac Dilation and Dysfunction in a New Dominant-Negative Transgenic Mouse Model.

Cells

Sun X,Zhou N,Ma B,Wu W,Stoll S,Lai L,Qin G,Qiu H

MA3-004 was used in Western Blot, Immunoprecipitation to determine the effects of impaired VCP activity on the heart.

Tue Oct 26 00:00:00 EDT 2021

Functional Inhibition of Valosin-Containing Protein Induces Cardiac Dilation and Dysfunction in a New Dominant-Negative Transgenic Mouse Model.

Cells

Sun X,Zhou N,Ma B,Wu W,Stoll S,Lai L,Qin G,Qiu H

MA3-004 was used in Western Blot, Immunoprecipitation to determine the effects of impaired VCP activity on the heart.

Tue Oct 26 00:00:00 EDT 2021

Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.

International journal of molecular sciences

Lee SH,Du J,Hwa J,Kim WH

MA3-004 was used in Western Blotting to investigate the expression of parkin within healthy and diabetic platelets, hearts, muscles and brains to find the significance of parkin in diabetes meticullus and its possible therapeutic use.

Sat Aug 15 00:00:00 EDT 2020

Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.

International journal of molecular sciences

Lee SH,Du J,Hwa J,Kim WH

Sat Aug 15 00:00:00 EDT 2020

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Cell chemical biology

Wu X,Spence JS,Das T,Yuan X,Chen C,Zhang Y,Li Y,Sun Y,Chandran K,Hang HC,Peng T

MA3-004 was used in Western Blotting to reveal the interaction of IFITM3 with VCP and uncovered key functional roles of the interaction on modulating IFITM3 trafficking.

Thu May 21 00:00:00 EDT 2020

ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.

Molecular cell

Wang B,Maxwell BA,Joo JH,Gwon Y,Messing J,Mishra A,Shaw TI,Ward AL,Quan H,Sakurada SM,Pruett-Miller SM,Bertorini T,Vogel P,Kim HJ,Peng J,Taylor JP,Kundu M

Published figure using VCP monoclonal antibody (Product # MA3-004) in Western Blot

Thu May 16 00:00:00 EDT 2019

Parkin is a disease modifier in the mutant SOD1 mouse model of ALS.

EMBO molecular medicine

Palomo GM,Granatiero V,Kawamata H,Konrad C,Kim M,Arreguin AJ,Zhao D,Milner TA,Manfredi G

Published figure using VCP monoclonal antibody (Product # MA3-004) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

Parkin is a disease modifier in the mutant SOD1 mouse model of ALS.

EMBO molecular medicine

Palomo GM,Granatiero V,Kawamata H,Konrad C,Kim M,Arreguin AJ,Zhao D,Milner TA,Manfredi G

Published figure using VCP monoclonal antibody (Product # MA3-004) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

UBXD1 is a mitochondrial recruitment factor for p97/VCP and promotes mitophagy.

Scientific reports

Bento AC,Bippes CC,Kohler C,Hemion C,Frank S,Neutzner A

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Aug 17 00:00:00 EDT 2018

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.

The EMBO journal

Mateju D,Franzmann TM,Patel A,Kopach A,Boczek EE,Maharana S,Lee HO,Carra S,Hyman AA,Alberti S

MA3004 was used in immunocytochemistry to characterize the components of stress granules

Wed Jun 14 00:00:00 EDT 2017

Molecular neurodegeneration

Ash PEA,Stanford EA,Al Abdulatif A,Ramirez-Cardenas A,Ballance HI,Boudeau S,Jeh A,Murithi JM,Tripodis Y,Murphy GJ,Sherr DH,Wolozin B

MA3-004 was used in Western Blotting to suggest that environmental dioxin induced aryl hydrocarbon receptor activation increases ALS pathological protein TDP-43 concentration in human neuronal cell lines and mouse brain.

Fri May 05 00:00:00 EDT 2017

The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress.

The American journal of pathology

Rodriguez-Ortiz CJ,Flores JC,Valenzuela JA,Rodriguez GJ,Zumkehr J,Tran DN,Kimonis VE,Kitazawa M

MA3-004 was used in immunocytochemistry and western blot to research delayed stress granule resolution on oxidative stress from myoblast C2C12 transfected with mutant valosin-containing protein

Wed Jun 01 00:00:00 EDT 2016

Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans.

Neuropathology : official journal of the Japanese Society of Neuropathology

Ito M,Nakamura K,Mori F,Miki Y,Tanji K,Wakabayashi K

MA3-004 was used in immunohistochemistry - paraffin section to examine eosinophilic neuronal cytoplasmic inclusions

Sat Oct 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.

The Journal of biological chemistry

Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunofluorescence

Fri Jul 01 00:00:00 EDT 2016

The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction.

eLife

Koizumi S,Irie T,Hirayama S,Sakurai Y,Yashiroda H,Naguro I,Ichijo H,Hamazaki J,Murata S

MA3-004 was used in western blot demonstrate that DNA-damage inducible 1 homolog 2 is required to cleave and activate Nrf1

Tue Aug 16 00:00:00 EDT 2016

PFKM gene defect and glycogen storage disease GSDVII with misleading enzyme histochemistry.

Neurology. Genetics

Auranen M,Palmio J,Ylikallio E,Huovinen S,Paetau A,Sandell S,Haapasalo H,Viitaniemi K,Piirilä P,Tyynismaa H,Udd B

MA3-004 was used in Immunohistochemistry to suggest that glycogen storage disease type VII cannot be excluded by normal enzyme histochemistry previously used as the gold standard for diagnosis.

Mon Jun 01 00:00:00 EDT 2015

Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Neuropathology : official journal of the Japanese Society of Neuropathology

Mori F,Watanabe Y,Miki Y,Tanji K,Odagiri S,Eto K,Wakabayashi K

MA3-004 was used in immunohistochemistry to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases

Tue Apr 01 00:00:00 EDT 2014

Eukaryotic stress granules are cleared by autophagy and Cdc48/VCP function.

Cell

Buchan JR,Kolaitis RM,Taylor JP,Parker R

MA3-004 was used in western blot to study the roles of autophagy and Cdc48/VCP in the clearance of eukaryotic stress granules and the significance for neurodegenerative disease

Thu Jun 20 00:00:00 EDT 2013

VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations.

Neuron

Kim NC,Tresse E,Kolaitis RM,Molliex A,Thomas RE,Alami NH,Wang B,Joshi A,Smith RB,Ritson GP,Winborn BJ,Moore J,Lee JY,Yao TP,Pallanck L,Kundu M,Taylor JP

MA3-004 was used in western blot to study the role of VCP in PIMK1/Parkin-dependent mitochondrial quality control in a Drosophila model of VCP-mediated degenerative disease

Wed Apr 10 00:00:00 EDT 2013

VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations.

Neuron

Kim NC,Tresse E,Kolaitis RM,Molliex A,Thomas RE,Alami NH,Wang B,Joshi A,Smith RB,Ritson GP,Winborn BJ,Moore J,Lee JY,Yao TP,Pallanck L,Kundu M,Taylor JP

MA3-004 was used in western blot to study the role of VCP in PIMK1/Parkin-dependent mitochondrial quality control in a Drosophila model of VCP-mediated degenerative disease

Wed Apr 10 00:00:00 EDT 2013

Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1.

Annals of neurology

Hackman P,Sarparanta J,Lehtinen S,Vihola A,Evilä A,Jonson PH,Luque H,Kere J,Screen M,Chinnery PF,Åhlberg G,Edström L,Udd B

MA3-004 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify the molecular cause of Welander distal myopathy (WDM), a classic autosomal dominant distal myopathy.

Mon Apr 01 00:00:00 EDT 2013

Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice.

The American journal of pathology

Rodriguez-Ortiz CJ,Hoshino H,Cheng D,Liu-Yescevitz L,Blurton-Jones M,Wolozin B,LaFerla FM,Kitazawa M

MA3-004 was used in immunohistochemistry and immunoprecipitation to study the effect of valosin-containing protein mutations on ubiquitin and TDP-43 accumulation and cognitive function in mice

Thu Aug 01 00:00:00 EDT 2013

Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice.

The American journal of pathology

Rodriguez-Ortiz CJ,Hoshino H,Cheng D,Liu-Yescevitz L,Blurton-Jones M,Wolozin B,LaFerla FM,Kitazawa M

MA3-004 was used in immunohistochemistry and immunoprecipitation to study the effect of valosin-containing protein mutations on ubiquitin and TDP-43 accumulation and cognitive function in mice

Thu Aug 01 00:00:00 EDT 2013

Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice.

The American journal of pathology

Rodriguez-Ortiz CJ,Hoshino H,Cheng D,Liu-Yescevitz L,Blurton-Jones M,Wolozin B,LaFerla FM,Kitazawa M

MA3-004 was used in immunohistochemistry and immunoprecipitation to study the effect of valosin-containing protein mutations on ubiquitin and TDP-43 accumulation and cognitive function in mice

Thu Aug 01 00:00:00 EDT 2013

Valosin-containing protein immunoreactivity in tauopathies, synucleinopathies, polyglutamine diseases and intranuclear inclusion body disease.

Neuropathology : official journal of the Japanese Society of Neuropathology

Mori F,Tanji K,Toyoshima Y,Sasaki H,Yoshida M,Kakita A,Takahashi H,Wakabayashi K

MA3-004 was used in immunohistochemistry to study the presence of structures containing VCP in the brains from 72 patients with a range of neurodegenerative diseases

Sun Dec 01 00:00:00 EST 2013

Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment.

Acta neuropathologica communications

Kuijpers M,van Dis V,Haasdijk ED,Harterink M,Vocking K,Post JA,Scheper W,Hoogenraad CC,Jaarsma D

MA3-004 was used in immunohistochemistry to determine ER quality control compartments created by amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions

Wed Jun 12 00:00:00 EDT 2013

Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment.

Acta neuropathologica communications

Kuijpers M,van Dis V,Haasdijk ED,Harterink M,Vocking K,Post JA,Scheper W,Hoogenraad CC,Jaarsma D

MA3-004 was used in immunohistochemistry to determine ER quality control compartments created by amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions

Wed Jun 12 00:00:00 EDT 2013

Different p97/VCP complexes function in retrotranslocation step of mammalian ER-associated degradation (ERAD).

The international journal of biochemistry & cell biology

Ballar P,Pabuccuoglu A,Kose FA

MA3-004 was used in western blot to investigate the role of different p97/VCP complexes in Hrd1- and gp78-mediated mammalian endoplasmic reticulum-associated degradation

Fri Apr 01 00:00:00 EDT 2011

Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis.

Reproductive biology and endocrinology : RB&E

Cayli S,Ocakli S,Erdemir F,Tas U,Aslan H,Yener T,Karaca Z

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunohistochemistry

Fri Aug 19 00:00:00 EDT 2011

Herp regulates Hrd1-mediated ubiquitylation in a ubiquitin-like domain-dependent manner.

The Journal of biological chemistry

Kny M,Standera S,Hartmann-Petersen R,Kloetzel PM,Seeger M

Published figure using VCP monoclonal antibody (Product # MA3-004) in Immunoprecipitation

Fri Feb 18 00:00:00 EST 2011

SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates.

The Journal of biological chemistry

Iida Y,Fujimori T,Okawa K,Nagata K,Wada I,Hosokawa N

MA3-004 was used in western blot to investigate the stability of mammalian HRD1-SEL1L complex and the regulatory machinery of its stability and assembly

Fri May 13 00:00:00 EDT 2011

VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.

Autophagy

Tresse E,Salomons FA,Vesa J,Bott LC,Kimonis V,Yao TP,Dantuma NP,Taylor JP

MA3-004 was used in immunocytochemistry and western blot to investigate the role of VCP/p97 on the maturation of autophagosome

Mon Feb 01 00:00:00 EST 2010

VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.

Autophagy

Tresse E,Salomons FA,Vesa J,Bott LC,Kimonis V,Yao TP,Dantuma NP,Taylor JP

MA3-004 was used in immunocytochemistry and western blot to investigate the role of VCP/p97 on the maturation of autophagosome

Mon Feb 01 00:00:00 EST 2010

Differential regulation of CFTRDeltaF508 degradation by ubiquitin ligases gp78 and Hrd1.

The international journal of biochemistry & cell biology

Ballar P,Ors AU,Yang H,Fang S

MA3-004 was used in western blot to investigate the role of gp78 and Hrd1 ubiquitin ligases in the degradation of cystic fibrosis transmembrane conductance regulator with the deletion of phenylalanine 508

Fri Jan 01 00:00:00 EST 2010

Mutation of FIG4 causes a rapidly progressive, asymmetric neuronal degeneration.

Brain : a journal of neurology

Zhang X,Chow CY,Sahenk Z,Shy ME,Meisler MH,Li J

MA3-004 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to describe a novel phenotype of rapidly progressive and asymmetric paralysis in two patients with mutations in FIG4.

Fri Aug 01 00:00:00 EDT 2008

Nuclear localization of valosin-containing protein in normal muscle and muscle affected by inclusion-body myositis.

Muscle & nerve

Greenberg SA,Watts GD,Kimonis VE,Amato AA,Pinkus JL

MA3-004 was used in immunohistochemistry to investigate the role of valosin-containing protein in inclusion-body myopathy with Paget disease and frontotemporal dementia

Mon Oct 01 00:00:00 EDT 2007

Selective inhibition of endoplasmic reticulum-associated degradation rescues DeltaF508-cystic fibrosis transmembrane regulator and suppresses interleukin-8 levels: therapeutic implications.

The Journal of biological chemistry

Vij N,Fang S,Zeitlin PL

MA3-004 was used in western blot to study the effect of endoplasmic reticulum-associated degradation inhibition on DeltaF508-cystic fibrosis transmembrane regulator and interleukin-8.

Fri Jun 23 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; or HEL-S-70; p97 atpase; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP; yeast Cdc48p homolog

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Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; TERA; VCP

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UniProt ID: (Human) P55072, (Mouse) Q01853, (Rat) P46462

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Entrez Gene ID: (Human) 7415, (Mouse) 269523, (Rat) 116643

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Function(s)

protein binding ATP binding lipid binding ATPase activity protein phosphatase binding protein domain specific binding polyubiquitin binding ubiquitin protein ligase binding deubiquitinase activator activity K48-linked polyubiquitin binding MHC class I protein binding identical protein binding ADP binding ubiquitin-like protein ligase binding poly(A) RNA binding BAT3 complex binding ubiquitin-specific protease binding nucleotide binding receptor binding hydrolase activity protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VCP Monoclonal Antibody (5)

VCP Antibody (MA3-004) in IP

Product Details

MA3-004

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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