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VDAC/Porin Antibody (600-401-882-RTU) in WB
Western Blot Results of Ready to Use Rabbit Anti-VDAC/Porin Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK293T WC Lysate. Lane 3: HeLa WC Lysate (p/n W09-000-364). Load: 10µL. Primary Antibody: Ready to Use Rabbit Anti-VDAC1/Porin Antibody 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073). Expect: ~31 kDa.
Product Details
600-401-882-RTU
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Goat,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.01% BSA, 25% glycerol, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
RTU Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC constitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BR1-VDAC; Brain-derived voltage-dependent anion channel 1; hVDAC1; MGC111064; Mitochondrial Porin; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN-31-HL; rVDAC1; VD; VDAC-1; VDAC-5; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5
Gene Aliases: AL033343; PORIN; VDAC; VDAC-1; VDAC1; Vdac5
UniProt ID: (Bovine) P45879, (Human) P21796, (Pig) Q9MZ16, (Mouse) Q60932, (Rat) Q9Z2L0
Entrez Gene ID: (Bovine) 282119, (Human) 7416, (Pig) 397010, (Goat) 102173290, (Mouse) 22333, (Rat) 83529
anion transport
apoptotic process
inorganic anion transport
anion transmembrane transport
behavioral fear response
mitochondrial calcium ion transport
neuron-neuron synaptic transmission
learning
viral process
macroautophagy
epithelial cell differentiation
regulation of mitophagy
regulation of anion transmembrane transport
negative regulation of reactive oxygen species metabolic process
transport
ion transport
chemical synaptic transmission
regulation of anion transport
transmembrane transport
ion transmembrane transport
regulation of ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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