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VDR Antibody (PA1-711) in IHC
Frontiers in medicine 2021 -
Figure 5 Digital images for different cell type of adenocarcinoma from 3 patients. Left: moderate differentiated adenocarcinoma, stage III. Middle: poor differentiated adenocarcinoma, stage I. Right: poor differentiated adenocarcinoma, stage III. From the top to the bottom: H&E stain, VDR, Ki67, CK18 stain processed by scanner and QuPath software.
Product Details
PA1-711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
Avian,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: E(395) E H S K Q Y R C L S F Q P E C S M K(413)
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2212362
Product Specific Information
PA1-711 detects vitamin D receptor (VDR) from human, mouse and rat tissues.
PA1-711 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~53 kDa protein representing VDR in COS-7 cells transfected with the human gene. PA1-711 supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).
PA1-711 immunizing peptide corresponds to amino acid residues 395-413 from human VDR.
Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; Nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; VDR; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; Vitamin D3 receptor
Gene Aliases: NR1I1; PPP1R163; VDR
UniProt ID: (Human) P11473, (Chicken) O42392, (Rat) P13053, (Mouse) P48281
Entrez Gene ID: (Human) 7421, (Chicken) 395988, (Rat) 24873, (Mouse) 22337
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
protein binding
zinc ion binding
calcitriol receptor activity
lithocholic acid receptor activity
sequence-specific DNA binding
retinoid X receptor binding
vitamin D response element binding
calcitriol binding
lithocholic acid binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
vitamin D binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
skeletal system development
transcription initiation from RNA polymerase II promoter
calcium ion transport
cellular calcium ion homeostasis
signal transduction
lactation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of keratinocyte proliferation
positive regulation of vitamin D 24-hydroxylase activity
bile acid signaling pathway
steroid hormone mediated signaling pathway
positive regulation of keratinocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
decidualization
intestinal absorption
positive regulation of apoptotic process involved in mammary gland involution
regulation of calcidiol 1-monooxygenase activity
mammary gland branching involved in pregnancy
vitamin D receptor signaling pathway
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to vitamin D
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
heart development
aging
organ morphogenesis
intracellular receptor signaling pathway
response to estradiol
response to calcium ion
regulation of calcium ion transport
cellular response to vitamin D
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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