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VDR Monoclonal Antibody (1A9C1)

VDR Antibody in Immunocytochemistry (ICC/IF)

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VDR Antibody (67192-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67192-1-Ig (VDR antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Product Details

67192-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1A9C1

Immunogen

VDR Fusion Protein Ag28188

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vitamin D receptor (VDR) on the cell membrane of mouse macrophages participates in the formation of lipopolysaccharide tolerance: mVDR is related to the effect of artesunate to reverse LPS tolerance.

Cell communication and signaling : CCS

Zhang Y,Zhou J,Hua L,Li P,Wu J,Shang S,Deng F,Luo J,Liao M,Wang N,Pan X,Yuan Y,Zheng Y,Lu Y,Huang Y,Zheng J,Liu X,Li X,Zhou H

Mon May 29 00:00:00 EDT 2023

Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway.

Frontiers in pharmacology

Sun X,Chen Z,Yu L,Zeng W,Sun B,Fan H,Bai Y

Sun Jun 11 00:00:00 EDT 2023

Astragalus polysaccharide protects sepsis model rats after cecum ligation and puncture.

Frontiers in bioengineering and biotechnology

Li J,Zhao J,Chai Y,Li W,Liu X,Chen Y

Tue Nov 08 00:00:00 EST 2022

Claudin1 decrease induced by 1,25-dihydroxy-vitamin D3 potentiates gefitinib resistance therapy through inhibiting AKT activation-mediated cancer stem-like properties in NSCLC cells.

Cell death discovery

Jia Z,Wang K,Duan Y,Hu K,Zhang Y,Wang M,Xiao K,Liu S,Pan Z,Ding X

Fri Mar 18 00:00:00 EDT 2022

Asperuloside inhibited epithelial-mesenchymal transition in colitis associated cancer via activation of vitamin D receptor.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lu Y,Guan T,Xu S,Chen YE,Shen Q,Zhu S,Liu Y,Liang J,Hou S

Fri Jul 01 00:00:00 EDT 2022

EB1089 promotes the expression of vitamin D receptor in the intestinal epithelial cell line HT-29 and reduces lipopolysaccharide-induced inflammatory response.

Annals of translational medicine

Lu D,Yu M,Chen L,Ye J,Huang L,Zhu G,Lan B

Fri Apr 01 00:00:00 EDT 2022

Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling.

Journal of diabetes research

Yuan Q,Zhang R,Sun M,Guo X,Yang J,Bian W,Xie C,Miao D,Mao L

Tue Mar 29 00:00:00 EDT 2022

Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling.

Journal of diabetes research

Yuan Q,Zhang R,Sun M,Guo X,Yang J,Bian W,Xie C,Miao D,Mao L

Tue Mar 29 00:00:00 EDT 2022

Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling.

Journal of diabetes research

Yuan Q,Zhang R,Sun M,Guo X,Yang J,Bian W,Xie C,Miao D,Mao L

Tue Mar 29 00:00:00 EDT 2022

Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling.

Journal of diabetes research

Yuan Q,Zhang R,Sun M,Guo X,Yang J,Bian W,Xie C,Miao D,Mao L

Tue Mar 29 00:00:00 EDT 2022

Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis via Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which Is Regulated by METTL3 and YTHDF3.

Frontiers in microbiology

Zhu W,Zhang H,Wang S

Sat Apr 02 00:00:00 EDT 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Wed Nov 23 00:00:00 EST 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Wed Nov 23 00:00:00 EST 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Wed Nov 23 00:00:00 EST 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Wed Nov 23 00:00:00 EST 2022

TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts.

Biochimica et biophysica acta. Molecular cell research

Jin A,Tang X,Zhai W,Li Y,Sun Q,Liu L,Yang X,Ren H,Lu S

Wed Sep 01 00:00:00 EDT 2021

Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.

Frontiers in cell and developmental biology

Wang S,Wu T,Sun J,Li Y,Yuan Z,Sun W

Sat Jan 08 00:00:00 EST 2022

Bioinformatics

Protein Aliases: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; Nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; VDR; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; Vitamin D3 receptor

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Gene Aliases: NR1I1; PPP1R163; VDR

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UniProt ID: (Human) P11473, (Rat) P13053, (Mouse) P48281

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Entrez Gene ID: (Human) 7421, (Rat) 24873, (Mouse) 22337

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity protein binding zinc ion binding calcitriol receptor activity lithocholic acid receptor activity sequence-specific DNA binding retinoid X receptor binding vitamin D response element binding calcitriol binding lithocholic acid binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding vitamin D binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis skeletal system development transcription initiation from RNA polymerase II promoter calcium ion transport cellular calcium ion homeostasis signal transduction lactation negative regulation of cell proliferation positive regulation of gene expression negative regulation of keratinocyte proliferation positive regulation of vitamin D 24-hydroxylase activity bile acid signaling pathway steroid hormone mediated signaling pathway positive regulation of keratinocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization intestinal absorption positive regulation of apoptotic process involved in mammary gland involution regulation of calcidiol 1-monooxygenase activity mammary gland branching involved in pregnancy vitamin D receptor signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development heart development aging organ morphogenesis intracellular receptor signaling pathway response to estradiol response to calcium ion regulation of calcium ion transport cellular response to vitamin D apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VDR Monoclonal Antibody (1A9C1)

VDR Antibody (67192-1-IG) in ICC/IF

Product Details

67192-1-IG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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