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VDR Antibody (MA5-37585) in WB
Western Blot analysis of VDR using the VDR Monoclonal Antibody (Product # MA5-37585) at a 1:2000 dilution. Lane 1: 293 whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: MCF-7 whole cell lysate, Lane 5: MDA-MB-453 whole cell lysate, Lane 6: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
MA5-37585
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgA
Class
Monoclonal
Type
Antibody
Clone
517CT23.5.1
Immunogen
purified His-tagged VDR protein (fragment)
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897513
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; Nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; VDR; vitamin D nuclear receptor variant 1; vitamin D receptor; Vitamin D3 receptor
Gene Aliases: NR1I1; PPP1R163; VDR
UniProt ID: (Human) P11473
Entrez Gene ID: (Human) 7421
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
protein binding
zinc ion binding
calcitriol receptor activity
lithocholic acid receptor activity
sequence-specific DNA binding
retinoid X receptor binding
vitamin D response element binding
calcitriol binding
lithocholic acid binding
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
skeletal system development
transcription initiation from RNA polymerase II promoter
calcium ion transport
cellular calcium ion homeostasis
signal transduction
lactation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of keratinocyte proliferation
positive regulation of vitamin D 24-hydroxylase activity
bile acid signaling pathway
steroid hormone mediated signaling pathway
positive regulation of keratinocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
decidualization
intestinal absorption
positive regulation of apoptotic process involved in mammary gland involution
regulation of calcidiol 1-monooxygenase activity
mammary gland branching involved in pregnancy
vitamin D receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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