Invitrogen

VDR Monoclonal Antibody (9A7gamma.E10.E4)

1 Published Figure

8 References

View all (22) VDR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

VDR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

VDR Antibody (MA5-14617) in ICC/IF

Immunofluorescence analysis of VDR was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VDR (9A7gamma.E10.E4) Rat Monoclonal Antibody (Product # MA5-14617) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at... View More

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Product Details

MA5-14617

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Gel Shift (GS)

1 mg/mL

Product Specifications

Species Reactivity

Amphibian, Chicken, Fish, Hamster, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Sheep

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9A7gamma.E10.E4

Immunogen

Vitamin D receptor protein from chicken intestine

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10989184

Product Specific Information

MA5-14617 targets Vitamin D Receptor in GS and WB applications and shows reactivity with Amphibian, Chicken, Fish, Hamster, Human, mouse, Porcine, and Rat samples.

The MA5-14617 immunogen is vitamin D receptor protein from chicken intestine.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer.

Breast cancer research and treatment

Thakkar A,Wang B,Picon-Ruiz M,Buchwald P,Ince TA

Published figure using VDR monoclonal antibody (Product # MA5-14617) in Western Blot

Sun May 01 00:00:00 EDT 2016

Mitochondrial translocation of vitamin D receptor is mediated by the permeability transition pore in human keratinocyte cell line.

PloS one

Silvagno F,Consiglio M,Foglizzo V,Destefanis M,Pescarmona G

MA5-14617 was used in immunoprecipitation to study the role of the permeability transition pore in the mitochondrial translocation of the vitamin D receptor in human keratinocytes

Fri Jul 19 00:00:00 EDT 2013

Crosstalk between the peroxisome proliferator-activated receptor γ (PPARγ) and the vitamin D receptor (VDR) in human breast cancer cells: PPARγ binds to VDR and inhibits 1α,25-dihydroxyvitamin D3 mediated transactivation.

Experimental cell research

Alimirah F,Peng X,Yuan L,Mehta RR,von Knethen A,Choubey D,Mehta RG

MA5-14617 was used in western blot to study the functional consequences of PPAR-gamma-VDR cross-talk in human breast cancer cells

Thu Nov 15 00:00:00 EST 2012

Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.

Journal of animal science

Sidler-Lauff K,Boos A,Kraenzlin M,Liesegang A

MA5-14617 was used in immunohistochemistry to study the effect of Ca2+ concentration on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of growing goats

Mon Nov 01 00:00:00 EDT 2010

Chemotherapeutic effect of calcidiol derivative B3CD in a neuroblastoma xenograft model.

Chemical biology & drug design

Lange TS,Zou Y,Singh RK,Kim KK,Kristjansdottir K,Sholler GL,Brard L

MA5-14617 was used in western blot to investigate the effect of bromoacetoxy-calcidiol on neuroblastoma cells and its potential therapeutic applications in vivo

Sun Aug 01 00:00:00 EDT 2010

1,25 Dihydroxyvitamin D(3) receptor expression in superficial transitional cell carcinoma of the bladder: a possible prognostic factor?

European urology

Sahin MO,Canda AE,Yorukoglu K,Mungan MU,Sade M,Kirkali Z

MA5-14617 was used in immunohistochemistry to investigate the prognostic value of vitamin D receptors in superficial transitional cell carcinoma of the human bladder

Sat Jan 01 00:00:00 EST 2005

Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.

The Journal of steroid biochemistry and molecular biology

Narvaez CJ,Byrne BM,Romu S,Valrance M,Welsh J

MA5-14617 was used in western blot to investigate the effect of TPA on the apoptosis induced by 1,25-dihydroxyvitamin D3 in MCF-7 vitamin D3-resistant variant

Sat Feb 01 00:00:00 EST 2003

Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.

Molecular and cellular endocrinology

Zinser GM,McEleney K,Welsh J

MA5-14617 was used in western blot to characterize mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice

Fri Feb 28 00:00:00 EST 2003

Bioinformatics

Protein Aliases: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; Nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; VDR; vitamin D (1,25- dihydroxyvitamin D3) receptor; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; Vitamin D3 receptor

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Gene Aliases: I79_025795; NR1I1; PPP1R163; VDR

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UniProt ID: (Human) P11473, (Chicken) O42392, (Pig) A3RGC1, (Rat) P13053, (Mouse) P48281

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Entrez Gene ID: (Human) 7421, (Chicken) 395988, (Pig) 396628, (Rat) 24873, (Chinese hamster) 100767048, (Mouse) 22337

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity protein binding zinc ion binding calcitriol receptor activity lithocholic acid receptor activity sequence-specific DNA binding retinoid X receptor binding vitamin D response element binding calcitriol binding lithocholic acid binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding vitamin D binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis skeletal system development transcription initiation from RNA polymerase II promoter calcium ion transport cellular calcium ion homeostasis signal transduction lactation negative regulation of cell proliferation positive regulation of gene expression negative regulation of keratinocyte proliferation positive regulation of vitamin D 24-hydroxylase activity bile acid signaling pathway steroid hormone mediated signaling pathway positive regulation of keratinocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization intestinal absorption positive regulation of apoptotic process involved in mammary gland involution regulation of calcidiol 1-monooxygenase activity mammary gland branching involved in pregnancy vitamin D receptor signaling pathway regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to vitamin D transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development heart development aging organ morphogenesis intracellular receptor signaling pathway response to estradiol response to calcium ion regulation of calcium ion transport cellular response to vitamin D apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VDR Monoclonal Antibody (9A7gamma.E10.E4)

VDR Antibody (MA5-14617) in ICC/IF

Product Details

MA5-14617

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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