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VEGF-165 Antibody (500-P10-1MG) in WB
Western Blot: To detect Human VEGF 165 by Western Blot analysis VEGF-165 Polyclonal Antibody (Product # 500-P10-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P10-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Hamster,
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human VEGF 165
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929190
Product Specific Information
AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix.
Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF 165 (500-P10Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165.
Western Blot: To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF165; VPF
Gene Aliases: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) H3BLW7
Entrez Gene ID: (Human) 7422
fibronectin binding
cytokine activity
platelet-derived growth factor receptor binding
vascular endothelial growth factor receptor binding
protein binding
growth factor activity
heparin binding
chemoattractant activity
identical protein binding
protein homodimerization activity
vascular endothelial growth factor receptor 1 binding
vascular endothelial growth factor receptor 2 binding
protein heterodimerization activity
receptor agonist activity
extracellular matrix binding
growth factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
ovarian follicle development
patterning of blood vessels
vasculogenesis
response to hypoxia
in utero embryonic development
kidney development
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
positive regulation of neuroblast proliferation
positive regulation of mesenchymal cell proliferation
positive regulation of receptor internalization
platelet degranulation
positive regulation of leukocyte migration
heart morphogenesis
outflow tract morphogenesis
coronary vein morphogenesis
nervous system development
mesoderm development
lactation
positive regulation of cell proliferation
regulation of cell shape
positive regulation of endothelial cell migration
positive regulation of gene expression
monocyte differentiation
macrophage differentiation
lung development
positive regulation of cell migration
regulation of cGMP metabolic process
epithelial cell differentiation
positive regulation of vascular endothelial growth factor receptor signaling pathway
post-embryonic camera-type eye development
positive regulation of protein complex assembly
positive regulation of protein autophosphorylation
activation of protein kinase activity
positive regulation of CREB transcription factor activity
positive regulation of peptidyl-serine phosphorylation
tube formation
endothelial cell chemotaxis
cellular response to vascular endothelial growth factor stimulus
lymph vessel morphogenesis
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
VEGF-activated neuropilin signaling pathway
growth
eye photoreceptor cell development
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of apoptotic process
surfactant homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAP kinase activity
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of cell adhesion
positive regulation of transcription from RNA polymerase II promoter
vascular endothelial growth factor receptor signaling pathway
cell maturation
camera-type eye morphogenesis
cardiac muscle fiber development
branching morphogenesis of an epithelial tube
positive regulation of axon extension involved in axon guidance
artery morphogenesis
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of positive chemotaxis
induction of positive chemotaxis
positive regulation of cellular component movement
positive regulation of cell division
positive regulation of focal adhesion assembly
primitive erythrocyte differentiation
mammary gland alveolus development
cardiac vascular smooth muscle cell development
coronary artery morphogenesis
regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia
cellular response to hypoxia
dopaminergic neuron differentiation
commissural neuron axon guidance
positive regulation of protein kinase C signaling
positive regulation of cell migration involved in sprouting angiogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of retinal ganglion cell axon guidance
positive regulation of peptidyl-tyrosine autophosphorylation
positive regulation of p38MAPK cascade
positive regulation of histone deacetylase activity
positive regulation of retinal ganglion cell axon guidance
positive regulation of protein kinase D signaling
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