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Product Details
MA5-31315
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
105
Immunogen
Recombinant Human VEGF165/VEGFA protein (Met1-Arg191)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2786952
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human VEGF165/VEGFA.
Target Information
VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L-VEGF; MGC70609; RP1-261G23.1; VAS; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular endothelial growth factor isoform 165/endothelial-specific mitogen; Vascular permeability factor; Vasculotropin; VEGF; VEGF1; VEGFMGC70609; VPF; VPF ant
Gene Aliases: MVCD1; simVEGF165; VEGF; VEGFA; VPF
UniProt ID: (Human) H3BLW7
Entrez Gene ID: (Human) 7422, (Cynomolgus monkey) 102140241
fibronectin binding
cytokine activity
platelet-derived growth factor receptor binding
vascular endothelial growth factor receptor binding
protein binding
growth factor activity
heparin binding
chemoattractant activity
identical protein binding
protein homodimerization activity
vascular endothelial growth factor receptor 1 binding
vascular endothelial growth factor receptor 2 binding
protein heterodimerization activity
receptor agonist activity
extracellular matrix binding
growth factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
ovarian follicle development
patterning of blood vessels
vasculogenesis
response to hypoxia
in utero embryonic development
kidney development
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
positive regulation of neuroblast proliferation
positive regulation of mesenchymal cell proliferation
positive regulation of receptor internalization
platelet degranulation
positive regulation of leukocyte migration
heart morphogenesis
outflow tract morphogenesis
coronary vein morphogenesis
nervous system development
mesoderm development
lactation
positive regulation of cell proliferation
regulation of cell shape
positive regulation of endothelial cell migration
positive regulation of gene expression
monocyte differentiation
macrophage differentiation
lung development
positive regulation of cell migration
regulation of cGMP metabolic process
epithelial cell differentiation
positive regulation of vascular endothelial growth factor receptor signaling pathway
post-embryonic camera-type eye development
positive regulation of protein complex assembly
positive regulation of protein autophosphorylation
activation of protein kinase activity
positive regulation of CREB transcription factor activity
positive regulation of peptidyl-serine phosphorylation
tube formation
endothelial cell chemotaxis
cellular response to vascular endothelial growth factor stimulus
lymph vessel morphogenesis
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
VEGF-activated neuropilin signaling pathway
growth
eye photoreceptor cell development
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of apoptotic process
surfactant homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAP kinase activity
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of cell adhesion
positive regulation of transcription from RNA polymerase II promoter
vascular endothelial growth factor receptor signaling pathway
cell maturation
camera-type eye morphogenesis
cardiac muscle fiber development
branching morphogenesis of an epithelial tube
positive regulation of axon extension involved in axon guidance
artery morphogenesis
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of positive chemotaxis
induction of positive chemotaxis
positive regulation of cellular component movement
positive regulation of cell division
positive regulation of focal adhesion assembly
primitive erythrocyte differentiation
mammary gland alveolus development
cardiac vascular smooth muscle cell development
coronary artery morphogenesis
regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia
cellular response to hypoxia
dopaminergic neuron differentiation
commissural neuron axon guidance
positive regulation of protein kinase C signaling
positive regulation of cell migration involved in sprouting angiogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of retinal ganglion cell axon guidance
positive regulation of peptidyl-tyrosine autophosphorylation
positive regulation of p38MAPK cascade
positive regulation of histone deacetylase activity
positive regulation of retinal ganglion cell axon guidance
positive regulation of protein kinase D signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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