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Invitrogen
VEGF Receptor 1 (Flt1) Monoclonal Antibody (7A6), eBioscience™
-WB-20170216130641.jpg?time=20240507 "VEGF Receptor 1 (Flt1) Antibody in Western Blot (WB)")
FIGURE: 1 / 1
VEGF Receptor 1 (Flt1) Antibody (14-5936-82) in WB
Immunoblot analysis of non-boiled NP-40 lysates from Human Umbilical Vein Endothelial Cells (HUVEC) run on SDS-PAGE under non-reducing conditions. The membrane was probed with 2 µg/mL of Anti-Human VEGF Receptor 1 (Flt1) Purified followed by Anti-Rat IgG HRP.
-WB-20170216130641.jpg?time=20240507)
-WB-20170216130641.jpg?time=20240507?time=20240507 "VEGF Receptor 1 (Flt1) Antibody (14-5936-82) in WB")
Product Details
14-5936-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
7A6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1963640
Product Specific Information
Description: This 7A6 monoclonal antibody recognizes human VEGFR1 (vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT1). It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180 kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregnant women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including malignancies of hematopoietic origin.
Applications Reported: This 7A6 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 7A6 antibody has been tested by immunoblot analysis of HUVEC. It can be used at 1-5 µg/mL. This antibody only recognizes non-reduced epitopes. It is highly recommended to include protease inhibitors in the lysing buffer and to not boil lysates prior to separation by non-reducing SDS-PAGE.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FLT1 (VEGFR1) is a receptor tyrosine kinase which plays a critical role in angiogenesis. Active angiogenesis has been found to play important role in many pathological and physiological processes, such as tumor progression, diabetic retinopathy, rheumatoid arthritis and wound healing. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLT; FLT-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); i21; soluble VEGFR 1; sVEGFR 1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR-1
Gene Aliases: FLT; FLT-1; FLT1; FRT; VEGFR-1; VEGFR1
UniProt ID: (Human) P17948
Entrez Gene ID: (Human) 2321
transmembrane receptor protein tyrosine kinase activity
vascular endothelial growth factor-activated receptor activity
protein binding
ATP binding
growth factor binding
VEGF-A-activated receptor activity
VEGF-B-activated receptor activity
placental growth factor-activated receptor activity
transmembrane signal receptor
activation of MAPKK activity
angiogenesis
response to hypoxia
monocyte chemotaxis
regulation of smooth muscle contraction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
positive regulation of phospholipase C activity
positive regulation of phosphatidylinositol 3-kinase signaling
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
positive regulation of cell migration
intracellular receptor signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
cellular response to vascular endothelial growth factor stimulus
vascular endothelial growth factor receptor-1 signaling pathway
vascular endothelial growth factor signaling pathway
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of angiogenesis
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
blood vessel morphogenesis
embryonic morphogenesis
positive regulation of smooth muscle cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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