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VEGFB Antibody (500-P267BT-25UG) in ELISA
Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of VEGFB Polyclonal Antibody, Biotin (Product # 500-P267BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech VEGFB Polyclonal Antibody (Product # 500-P267-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B.
Product Details
500-P267BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human VEGF-B
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930156
Product Specific Information
AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B. Anti-Human VEGF-B-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF-B (500-P267) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B.
Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P267BT-1MG will be provided as 2 x 500 µg
Target Information
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF
Gene Aliases: VEGFB; VEGFL; VRF
UniProt ID: (Human) P49765
Entrez Gene ID: (Human) 7423
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