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VEGFD Antibody (MA5-36196) in ICC/IF
Immunocytochemical analysis of VEGFD in HUVEC cells using a VEGFD monoclonal antibody (Product #MA5-36196). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
Product Details
MA5-36196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-75
Immunogen
Recombinant protein within Human VEGFD aa 181-302
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2890517
Target Information
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-fos induced growth factor (vascular endothelial growth factor D); c-Fos-induced growth factor; FIGF; Vascular endothelial growth factor D; VEGF-D; VEGF4
Gene Aliases: AI325264; FIGF; VEGF-D; VEGFD
UniProt ID: (Human) O43915, (Mouse) P97946, (Rat) O35251
Entrez Gene ID: (Human) 2277, (Mouse) 14205, (Rat) 360457
platelet-derived growth factor receptor binding
vascular endothelial growth factor receptor binding
protein binding
growth factor activity
chemoattractant activity
protein homodimerization activity
vascular endothelial growth factor receptor 3 binding
vascular endothelial growth factor receptor 2 binding
growth factor
angiogenesis
response to hypoxia
platelet degranulation
cell proliferation
positive regulation of cell proliferation
regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of interleukin-6 production
vascular endothelial growth factor receptor signaling pathway
positive chemotaxis
induction of positive chemotaxis
positive regulation of cell division
positive regulation of mast cell chemotaxis
response to interleukin-1
dopaminergic neuron differentiation
cell differentiation
positive regulation of angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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