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Invitrogen
VGLUT1 Monoclonal Antibody (N28/9)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
VGLUT1 Antibody (MA5-27614) in WB
Western blot was performed using Anti-VGLUT1 Monoclonal Antibody (Product # MA5-27614) and a 50 kDa band corresponding to VGLUT1 was observed across tissues tested. Mouse Brain (Lane 1), Rat Brain (Lane 2) and Mouse Spleen (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chem... View More
Product Details
MA5-27614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N28/9
Immunogen
Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735410
Product Specific Information
1 µg/mL of MA5-27614 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 52kDa. No cross-reactivity against VGlut2.
This antibody was formerly sold as clone S28-9.
Target Information
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; solute carrier family 17 (vesicular glutamate transporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1
Gene Aliases: 2900052E22Rik; AI851913; BNPI; SLC17A7; VGLUT1
UniProt ID: (Human) Q9P2U7, (Rat) Q62634, (Mouse) Q3TXX4
Entrez Gene ID: (Human) 57030, (Rat) 116638, (Mouse) 72961
L-glutamate transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
extracellular-glutamate-gated chloride channel activity
sodium:inorganic phosphate symporter activity
sodium-dependent phosphate transmembrane transporter activity
symporter activity
secondary carrier transporter
neural retina development
ion transport
phosphate ion transport
neurotransmitter transport
brain development
long-term memory
glutamate secretion
synaptic transmission, glutamatergic
phosphate ion transmembrane transport
sodium ion transmembrane transport
sequestering of neurotransmitter
sodium-dependent phosphate transport
L-glutamate import
excitatory postsynaptic potential
L-glutamate transmembrane transport
synaptic vesicle lumen acidification
chloride transmembrane transport
chemical synaptic transmission
transport
sodium ion transport
response to stimulus
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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