Proteintech

VPRBP Polyclonal Antibody

VPRBP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

VPRBP Antibody (11612-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (Product # 11612-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

VPRBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VPRBP Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11612-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 36 publications 36 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPRBP Fusion Protein Ag2184 (707-1058 aa encoded by BC022792)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: WSQPLSALW GMKSVFDMKH SFTEDHYVEF SKHSQDRVIG TKGDIAHIYD IQTGNKLLTL FNPDLANNYK RNCATFNPTD DLVLNDGVLW DVRSAQAIHK FDKFNMNISG VFHPNGLEVI INTEIWDLRT FHLLHTVPAL DQCRVVFNHT GTVMYGAMLQ ADDEDDLMEE RMKSPFGSSF RTFNATDYKP IATIDVKRNI FDLCTDTKDC YLAVIENQGS MDALNMDTVC RLYEVGRQRL AEDEDEEEDQ EEEEQEEEDD DEDDDDTDDL DELDTDQLLE AELEEDDNNE NAGEDGDNDF SPSDEELANL LEEGEDGEDE DSDADEEVEL ILGDTDSSDN SDLEDDIILS LNE (707-1058 aa encoded by B C022792)

Target Information

Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex. The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition. Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors. Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2. In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. The HIV-1 Vpr protein hijacks the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex to promote ubiquitination and degradation of proteins such as TERT and ZIP/ZGPAT. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer.

Nature communications

Ghate NB,Kim S,Shin Y,Kim J,Doche M,Valena S,Situ A,Kim S,Rhie SK,Lenz HJ,Ulmer TS,Mumenthaler SM,An W

Mon Apr 17 00:00:00 EDT 2023

HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages.

bioRxiv : the preprint server for biology

Virgilio MC,Disbennett WM,Chen T,Lubow J,Welch JD,Collins KL

Tue Mar 21 00:00:00 EDT 2023

HIV-1 Vpr-induced DNA damage activates NF-κB independent of cell cycle arrest and CRL4A^DCAF1 engagement.

bioRxiv : the preprint server for biology

Sandoval C,Fregoso OI

Tue May 23 00:00:00 EDT 2023

Stabilization of SAMHD1 by NONO is crucial for Ara-C resistance in AML.

Cell death & disease

Zhang F,Sun J,Tang X,Liang Y,Jiao Q,Yu B,Dai Z,Yuan X,Li J,Yan J,Zhang Z,Fan S,Wang M,Hu H,Zhang C,Lv XB

Fri Jul 08 00:00:00 EDT 2022

Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation.

Nature communications

Arroyo M,Hastert FD,Zhadan A,Schelter F,Zimbelmann S,Rausch C,Ludwig AK,Carell T,Cardoso MC

Fri Sep 02 00:00:00 EDT 2022

Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation.

Nature communications

Arroyo M,Hastert FD,Zhadan A,Schelter F,Zimbelmann S,Rausch C,Ludwig AK,Carell T,Cardoso MC

Fri Sep 02 00:00:00 EDT 2022

Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation.

Nature communications

Arroyo M,Hastert FD,Zhadan A,Schelter F,Zimbelmann S,Rausch C,Ludwig AK,Carell T,Cardoso MC

Fri Sep 02 00:00:00 EDT 2022

HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells.

Cell reports

Reuschl AK,Mesner D,Shivkumar M,Whelan MVX,Pallett LJ,Guerra-Assunção JA,Madansein R,Dullabh KJ,Sigal A,Thornhill JP,Herrera C,Fidler S,Noursadeghi M,Maini MK,Jolly C

Tue Apr 12 00:00:00 EDT 2022

Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages.

Viruses

Murakami T,Matsuura R,Chutiwitoonchai N,Takei M,Aida Y

Fri Nov 19 00:00:00 EST 2021

The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.

PLoS biology

Dar AA,Sawada K,Dybas JM,Moser EK,Lewis EL,Park E,Fazelinia H,Spruce LA,Ding H,Seeholzer SH,Oliver PM

Mon Feb 01 00:00:00 EST 2021

The CRL4VPRBP(DCAF1) E3 ubiquitin ligase directs constitutive RAG1 degradation in a non-lymphoid cell line.

PloS one

Schabla NM,Swanson PC

Thu Nov 25 00:00:00 EST 2021

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes.

mSystems

Dybas JM,Lum KK,Kulej K,Reyes ED,Lauman R,Charman M,Purman CE,Steinbock RT,Grams N,Price AM,Mendoza L,Garcia BA,Weitzman MD

Tue Aug 31 00:00:00 EDT 2021

VprBP directs epigenetic gene silencing through histone H2A phosphorylation in colon cancer.

Molecular oncology

Ghate NB,Kim S,Spiller E,Kim S,Shin Y,Rhie SK,Smbatyan G,Lenz HJ,Mumenthaler SM,An W

Fri Oct 01 00:00:00 EDT 2021

DCAF1 regulates Treg senescence via the ROS axis during immunological aging.

The Journal of clinical investigation

Guo Z,Wang G,Wu B,Chou WC,Cheng L,Zhou C,Lou J,Wu D,Su L,Zheng J,Ting JP,Wan YY

Mon Nov 02 00:00:00 EST 2020

DCAF1 regulates Treg senescence via the ROS axis during immunological aging.

The Journal of clinical investigation

Guo Z,Wang G,Wu B,Chou WC,Cheng L,Zhou C,Lou J,Wu D,Su L,Zheng J,Ting JP,Wan YY

Mon Nov 02 00:00:00 EST 2020

The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma.

Cancers

Lyons Rimmer J,Ercolano E,Baiz D,Makhija M,Berger A,Sells T,Stroud S,Hilton D,Adams CL,Hanemann CO

Tue Jun 30 00:00:00 EDT 2020

Vpr Targets TET2 for Degradation by CRL4^VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.

Molecular cell

Lv L,Wang Q,Xu Y,Tsao LC,Nakagawa T,Guo H,Su L,Xiong Y

Thu Jun 07 00:00:00 EDT 2018

Vpr Targets TET2 for Degradation by CRL4^VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.

Molecular cell

Lv L,Wang Q,Xu Y,Tsao LC,Nakagawa T,Guo H,Su L,Xiong Y

Thu Jun 07 00:00:00 EDT 2018

VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.

Journal of immunology (Baltimore, Md. : 1950)

Schabla NM,Perry GA,Palmer VL,Swanson PC

Wed Aug 01 00:00:00 EDT 2018

VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.

Journal of immunology (Baltimore, Md. : 1950)

Schabla NM,Perry GA,Palmer VL,Swanson PC

Wed Aug 01 00:00:00 EDT 2018

HIV-1 Vpr hijacks EDD-DYRK2-DDB1^DCAF1 to disrupt centrosome homeostasis.

The Journal of biological chemistry

Hossain D,Ferreira Barbosa JA,Cohen ÉA,Tsang WY

Fri Jun 15 00:00:00 EDT 2018

HIV-1 Vpr hijacks EDD-DYRK2-DDB1^DCAF1 to disrupt centrosome homeostasis.

The Journal of biological chemistry

Hossain D,Ferreira Barbosa JA,Cohen ÉA,Tsang WY

Fri Jun 15 00:00:00 EDT 2018

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Nature microbiology

Yurkovetskiy L,Guney MH,Kim K,Goh SL,McCauley S,Dauphin A,Diehl WE,Luban J

Sat Dec 01 00:00:00 EST 2018

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Nature microbiology

Yurkovetskiy L,Guney MH,Kim K,Goh SL,McCauley S,Dauphin A,Diehl WE,Luban J

Sat Dec 01 00:00:00 EST 2018

Primate lentiviruses use at least three alternative strategies to suppress NF-κB-mediated immune activation.

PLoS pathogens

Hotter D,Krabbe T,Reith E,Gawanbacht A,Rahm N,Ayouba A,Van Driessche B,Van Lint C,Peeters M,Kirchhoff F,Sauter D

Tue Aug 01 00:00:00 EDT 2017

Primate lentiviruses use at least three alternative strategies to suppress NF-κB-mediated immune activation.

PLoS pathogens

Hotter D,Krabbe T,Reith E,Gawanbacht A,Rahm N,Ayouba A,Van Driessche B,Van Lint C,Peeters M,Kirchhoff F,Sauter D

Tue Aug 01 00:00:00 EDT 2017

The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.

Cell reports

Furukawa KT,Yamashita K,Sakurai N,Ohno S

Tue Aug 08 00:00:00 EDT 2017

The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.

Cell reports

Furukawa KT,Yamashita K,Sakurai N,Ohno S

Tue Aug 08 00:00:00 EDT 2017

The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.

Cell reports

Furukawa KT,Yamashita K,Sakurai N,Ohno S

Tue Aug 08 00:00:00 EDT 2017

The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.

Cell reports

Furukawa KT,Yamashita K,Sakurai N,Ohno S

Tue Aug 08 00:00:00 EDT 2017

TET1 inhibits cell proliferation by inducing RASSF5 expression.

Oncotarget

Li BT,Yu C,Xu Y,Liu SB,Fan HY,Pan WW

Tue Oct 17 00:00:00 EDT 2017

TET1 inhibits cell proliferation by inducing RASSF5 expression.

Oncotarget

Li BT,Yu C,Xu Y,Liu SB,Fan HY,Pan WW

Tue Oct 17 00:00:00 EDT 2017

TET1 inhibits cell proliferation by inducing RASSF5 expression.

Oncotarget

Li BT,Yu C,Xu Y,Liu SB,Fan HY,Pan WW

Tue Oct 17 00:00:00 EDT 2017

TET1 inhibits cell proliferation by inducing RASSF5 expression.

Oncotarget

Li BT,Yu C,Xu Y,Liu SB,Fan HY,Pan WW

Tue Oct 17 00:00:00 EDT 2017

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

Nature communications

Guo Z,Kong Q,Liu C,Zhang S,Zou L,Yan F,Whitmire JK,Xiong Y,Chen X,Wan YY

Tue Jan 05 00:00:00 EST 2016

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

Nature communications

Guo Z,Kong Q,Liu C,Zhang S,Zou L,Yan F,Whitmire JK,Xiong Y,Chen X,Wan YY

Tue Jan 05 00:00:00 EST 2016

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

Nature communications

Guo Z,Kong Q,Liu C,Zhang S,Zou L,Yan F,Whitmire JK,Xiong Y,Chen X,Wan YY

Tue Jan 05 00:00:00 EST 2016

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

Nature communications

Guo Z,Kong Q,Liu C,Zhang S,Zou L,Yan F,Whitmire JK,Xiong Y,Chen X,Wan YY

Tue Jan 05 00:00:00 EST 2016

Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.

Cell host & microbe

Kyei GB,Cheng X,Ramani R,Ratner L

Wed Jan 14 00:00:00 EST 2015

Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.

Cell host & microbe

Kyei GB,Cheng X,Ramani R,Ratner L

Wed Jan 14 00:00:00 EST 2015

Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.

Cell host & microbe

Kyei GB,Cheng X,Ramani R,Ratner L

Wed Jan 14 00:00:00 EST 2015

Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.

Cell host & microbe

Kyei GB,Cheng X,Ramani R,Ratner L

Wed Jan 14 00:00:00 EST 2015

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.

Molecular cell

Nakagawa T,Lv L,Nakagawa M,Yu Y,Yu C,D'Alessio AC,Nakayama K,Fan HY,Chen X,Xiong Y

Thu Jan 22 00:00:00 EST 2015

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.

Molecular cell

Nakagawa T,Lv L,Nakagawa M,Yu Y,Yu C,D'Alessio AC,Nakayama K,Fan HY,Chen X,Xiong Y

Thu Jan 22 00:00:00 EST 2015

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.

Molecular cell

Nakagawa T,Lv L,Nakagawa M,Yu Y,Yu C,D'Alessio AC,Nakayama K,Fan HY,Chen X,Xiong Y

Thu Jan 22 00:00:00 EST 2015

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.

Molecular cell

Nakagawa T,Lv L,Nakagawa M,Yu Y,Yu C,D'Alessio AC,Nakayama K,Fan HY,Chen X,Xiong Y

Thu Jan 22 00:00:00 EST 2015

Vpr Promotes Macrophage-Dependent HIV-1 Infection of CD4+ T Lymphocytes.

PLoS pathogens

Collins DR,Lubow J,Lukic Z,Mashiba M,Collins KL

Wed Jul 01 00:00:00 EDT 2015

Vpr Promotes Macrophage-Dependent HIV-1 Infection of CD4+ T Lymphocytes.

PLoS pathogens

Collins DR,Lubow J,Lukic Z,Mashiba M,Collins KL

Wed Jul 01 00:00:00 EDT 2015

Degradation of SAMHD1 by Vpx Is Independent of Uncoating.

Journal of virology

Jáuregui P,Logue EC,Schultz ML,Fung S,Landau NR

Fri May 01 00:00:00 EDT 2015

Degradation of SAMHD1 by Vpx Is Independent of Uncoating.

Journal of virology

Jáuregui P,Logue EC,Schultz ML,Fung S,Landau NR

Fri May 01 00:00:00 EDT 2015

Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1.

The Biochemical journal

Li J,Xu F,Hu S,Zhou J,Mei S,Zhao X,Cen S,Jin Q,Liang C,Guo F

Mon Jun 01 00:00:00 EDT 2015

Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1.

The Biochemical journal

Li J,Xu F,Hu S,Zhou J,Mei S,Zhao X,Cen S,Jin Q,Liang C,Guo F

Mon Jun 01 00:00:00 EDT 2015

Tumor suppressor protein Lgl mediates G1 cell cycle arrest at high cell density by forming an Lgl-VprBP-DDB1 complex.

Molecular biology of the cell

Yamashita K,Ide M,Furukawa KT,Suzuki A,Hirano H,Ohno S

Wed Jul 01 00:00:00 EDT 2015

Tumor suppressor protein Lgl mediates G1 cell cycle arrest at high cell density by forming an Lgl-VprBP-DDB1 complex.

Molecular biology of the cell

Yamashita K,Ide M,Furukawa KT,Suzuki A,Hirano H,Ohno S

Wed Jul 01 00:00:00 EDT 2015

CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation.

Nature communications