Proteintech

VPS34 (C terminal) Polyclonal Antibody

VPS34 (C terminal) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

VPS34 (C terminal) Antibody (12452-1-AP) in ICC/IF

Immunofluorescent analysis of PC-3 cells using 12452-1-AP (VPS34 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

VPS34 (C terminal) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VPS34 (C terminal) Antibody in Western Blot (WB)

Product Image Gallery

Product Details

12452-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 42 publications 42 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS34 (C terminal) Fusion Protein Ag3110 (600-887 aa encoded by BC033004)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KIRGIIPET ATLFKSALMP AQLFFKTEDG GKYPVIFKHG DDLRQDQLIL QIISLMDKLL RKENLDLKLT PYKVLATSTK HGFMQFIQSV PVAEVLDTEG SIQNFFRKYA PSENGPNGIS AEVMDTYVKS CAGYCVITYI LGVGDRHLDN LLLTKTGKLF HIDFGYILGR DPKPLPPPMK LNKEMVEGMG GTQSEQYQEF RKQCYTAFLH LRRYSNLILN LFSLMVDANI PDIALEPDKT VKKVQDKFRL DLSDEEAVHY MQSLIDESVH ALFAAVVEQI HKFAQYWRK (600-887 aa encoded by BC033004)

Target Information

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rosuvastatin promotes survival of random skin flaps through AMPK-mTOR pathway-induced autophagy.

International immunopharmacology

Ye H,Li F,Shen Y,Wu X,Zhao L,Zhang H,Yang J,Shui X

Mon May 01 00:00:00 EDT 2023

Sinomenine promotes flap survival by upregulating eNOS and eNOS-mediated autophagy via PI3K/AKT pathway.

International immunopharmacology

Zhang D,Jin C,Han T,Chen J,Ali Raza M,Li B,Wang L,Yan H

Wed Mar 01 00:00:00 EST 2023

Echovirus induces autophagy to promote viral replication via regulating mTOR/ULK1 signaling pathway.

Frontiers in immunology

Wu C,Zeng L,Yi W,Miao Y,Liu Y,Wang Q,Liu S,Peng G,Zheng Z,Xia J

Mon May 01 00:00:00 EDT 2023

Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization.

Journal of neuroinflammation

Zhang H,Chen Y,Li F,Wu C,Cai W,Ye H,Su H,He M,Yang L,Wang X,Zhou K,Ni W

Sat Jan 07 00:00:00 EST 2023

Piperine attenuates the inflammation, oxidative stress, and pyroptosis to facilitate recovery from spinal cord injury via autophagy enhancement.

Phytotherapy research : PTR

Zhang H,Wu C,Yu DD,Su H,Chen Y,Ni W

Wed Feb 01 00:00:00 EST 2023

Cyclic helix B peptide promotes random-pattern skin flap survival via TFE3-mediated enhancement of autophagy and reduction of ROS levels.

British journal of pharmacology

Lou J,Zhang H,Qi J,Xu Y,Wang X,Jiang J,Hu X,Ni L,Cai Y,Wang X,Gao W,Xiao J,Zhou K

Sat Jan 01 00:00:00 EST 2022

Paraquat Inhibits Autophagy Via Intensifying the Interaction Between HMGB1 and α-Synuclein.

Neurotoxicity research

Wang K,Zhang B,Zhang B,Wu K,Tian T,Yan W,Huang M

Fri Apr 01 00:00:00 EDT 2022

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress.

Autophagy

Yan JM,Zhang WK,Yan LN,Jiao YJ,Zhou CM,Yu XJ

Fri Jul 01 00:00:00 EDT 2022

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Cell insight

Zhou C,Dong X,Wang M,Qian X,Hu M,Liang K,Liang Y,Zhang R,Huang Y,Lyu H,Xiao S,Tang Y,Ali DW,Michalak M,Chen XZ,Tang J

Mon Aug 01 00:00:00 EDT 2022

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Cell insight

Zhou C,Dong X,Wang M,Qian X,Hu M,Liang K,Liang Y,Zhang R,Huang Y,Lyu H,Xiao S,Tang Y,Ali DW,Michalak M,Chen XZ,Tang J

Mon Aug 01 00:00:00 EDT 2022

Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma.

Cell death & disease

Jia X,Xin M,Xu J,Xiang X,Li X,Jiao Y,Wang L,Jiang J,Pang F,Zhang X,Zhang J

Tue Aug 30 00:00:00 EDT 2022

An Experimental Study Reveals the Protective Effect of Autophagy against Realgar-Induced Liver Injury via Suppressing ROS-Mediated NLRP3 Inflammasome Pathway.

International journal of molecular sciences

Yang J,Li J,Guo H,Zhang Y,Guo Z,Liu Y,Huo T

Thu May 19 00:00:00 EDT 2022

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps.

Frontiers in pharmacology

Dong C,Chen Z,Zhu L,Bsoul N,Wu H,Jiang J,Chen X,Lai Y,Yu G,Gu Y,Guo X,Gao W

Sat Mar 05 00:00:00 EST 2022

SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores.

Autophagy

Lee JE,Kim N,Jung M,Mun JY,Yoo JY

Fri Jul 01 00:00:00 EDT 2022

GDF-11 Protects the Traumatically Injured Spinal Cord by Suppressing Pyroptosis and Necroptosis via TFE3-Mediated Autophagy Augmentation.

Oxidative medicine and cellular longevity

Xu Y,Hu X,Li F,Zhang H,Lou J,Wang X,Wang H,Yin L,Ni W,Kong J,Wang X,Li Y,Zhou K,Xu H

Mon Feb 07 00:00:00 EST 2022

IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.

Nature communications

Hu F,Song D,Yan Y,Huang C,Shen C,Lan J,Chen Y,Liu A,Wu Q,Sun L,Xu F,Hu F,Chen L,Luo X,Feng Y,Huang S,Hu J,Wang G

Tue Jun 15 00:00:00 EDT 2021

Protective autophagy attenuates soft substrate-induced apoptosis through ROS/JNK signaling pathway in breast cancer cells.

Free radical biology & medicine

Chen Y,Li P,Peng Y,Xie X,Zhang Y,Jiang Y,Li T,Qin X,Li S,Yang H,Wu C,Zheng C,Zhu J,You F,Liu Y

Fri Aug 20 00:00:00 EDT 2021

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Nature communications

Wang W,Li J,Tan J,Wang M,Yang J,Zhang ZM,Li C,Basnakian AG,Tang HW,Perrimon N,Zhou Q

Wed Jan 20 00:00:00 EST 2021

Curcumin promotes the survival of ischemic random skin flaps via autophagy.

American journal of translational research

Tu Z,Jiang X,Li Y,Yang S,Lin D,Shi Y,Mao C,Zhang X,Lin C

Tue Apr 13 00:00:00 EDT 2021

Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.

Journal of cellular physiology

Li J,Chen H,Lou J,Bao G,Wu C,Lou Z,Wang X,Ding J,Li Z,Xiao J,Xu H,Gao W,Zhou K

Sat May 01 00:00:00 EDT 2021

Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.

Cancer letters

Zuo Q,Liao L,Yao ZT,Liu YP,Wang DK,Li SJ,Yin XF,He QY,Xu WW

Thu Sep 09 00:00:00 EDT 2021

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Developmental cell

Lei Y,Zhang X,Xu Q,Liu S,Li C,Jiang H,Lin H,Kong E,Liu J,Qi S,Li H,Xu W,Lu K

Mon Aug 23 00:00:00 EDT 2021

Pseudoginsenoside F11 Enhances the Viability of Random-Pattern Skin Flaps by Promoting TFEB Nuclear Translocation Through AMPK-mTOR Signal Pathway.

Frontiers in pharmacology

Zhou F,Zhang X,Jiang L,Li S,Chen Y,Wu J

Tue May 18 00:00:00 EDT 2021

Autophagy Alters Bladder Angiogenesis and Improves Bladder Hyperactivity in the Pathogenesis of Ketamine-Induced Cystitis in a Rat Model.

Biology

Lu JH,Wu YH,Juan TJ,Lin HY,Lin RJ,Chueh KS,Lee YC,Chang CY,Juan YS

Sun May 30 00:00:00 EDT 2021

Liraglutide, a TFEB-Mediated Autophagy Agonist, Promotes the Viability of Random-Pattern Skin Flaps.

Oxidative medicine and cellular longevity

Zhu X,Hu X,Lou J,Li J,Xu Y,Yu G,Wu C,Ding J,Gao W,Xiao J,Zhou K,Jia C

Fri May 28 00:00:00 EDT 2021

Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.

Nature communications

Tremel S,Ohashi Y,Morado DR,Bertram J,Perisic O,Brandt LTL,von Wrisberg MK,Chen ZA,Maslen SL,Kovtun O,Skehel M,Rappsilber J,Lang K,Munro S,Briggs JAG,Williams RL

Wed Mar 10 00:00:00 EST 2021

OAB-14 Effectively Ameliorates the Dysfunction of the Endosomal-Autophagic-Lysosomal Pathway in APP/PS1 Transgenic Mice.

ACS chemical neuroscience

Guo X,Bao X,Wang X,Liu D,Liu P,Chi T,Ji X,Zheng Z,Chen G,Zou L

Wed Nov 03 00:00:00 EDT 2021

VPS34 regulates dynamin to determine the endocytosis of mitochondria-targeted zinc oxide nanoparticles in human osteosarcoma cells.

Journal of materials chemistry. B

Pan X,He G,Hai B,Liu Y,Bian L,Yong L,Zhang H,Yang C,Du C,Mao T,Ma Y,Jia F,Dou X,Zhai S,Liu X

Sun Mar 21 00:00:00 EDT 2021

VPS34 regulates dynamin to determine the endocytosis of mitochondria-targeted zinc oxide nanoparticles in human osteosarcoma cells.

Journal of materials chemistry. B

Pan X,He G,Hai B,Liu Y,Bian L,Yong L,Zhang H,Yang C,Du C,Mao T,Ma Y,Jia F,Dou X,Zhai S,Liu X

Sun Mar 21 00:00:00 EDT 2021

The effects of mTOR or Vps34-mediated autophagy on methylmercury-induced neuronal apoptosis in rat cerebral cortex.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ni L,Wei Y,Pan J,Li X,Xu B,Deng Y,Yang T,Liu W

Wed Sep 01 00:00:00 EDT 2021

Betulinic acid inhibits pyroptosis in spinal cord injury by augmenting autophagy via the AMPK-mTOR-TFEB signaling pathway.

International journal of biological sciences

Wu C,Chen H,Zhuang R,Zhang H,Wang Y,Hu X,Xu Y,Li J,Li Y,Wang X,Xu H,Ni W,Zhou K

Fri Jan 21 00:00:00 EST 2022

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Theranostics

Zhou K,Zheng Z,Li Y,Han W,Zhang J,Mao Y,Chen H,Zhang W,Liu M,Xie L,Zhang H,Xu H,Xiao J

Fri May 28 00:00:00 EDT 2021

Butylphthalide Inhibits Autophagy and Promotes Multiterritory Perforator Flap Survival.

Frontiers in pharmacology

Li B,Chen Z,Luo X,Zhang C,Chen H,Wang S,Zhao M,Ma H,Liu J,Cheng M,Yang Y,Yan H

Tue Feb 16 00:00:00 EST 2021

Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement.

Frontiers in pharmacology

Wu C,Xu H,Li J,Hu X,Wang X,Huang Y,Li Y,Sheng S,Wang Y,Xu H,Ni W,Zhou K

Sat Apr 16 00:00:00 EDT 2022

LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes.

Molecular cancer

Zhou C,Yi C,Yi Y,Qin W,Yan Y,Dong X,Zhang X,Huang Y,Zhang R,Wei J,Ali DW,Michalak M,Chen XZ,Tang J

Wed Jul 29 00:00:00 EDT 2020

Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.

Journal of thrombosis and haemostasis : JTH

Bertović I,Kurelić R,Milošević I,Bender M,Krauss M,Haucke V,Jurak Begonja A

Wed Jul 01 00:00:00 EDT 2020

Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.

Journal of thrombosis and haemostasis : JTH

Bertović I,Kurelić R,Milošević I,Bender M,Krauss M,Haucke V,Jurak Begonja A

Wed Jul 01 00:00:00 EDT 2020

Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.

American journal of translational research

Zhang C,Shao Z,Chen Z,Lin C,Hu S,Lou Z,Li J,Zheng X,Lin N,Gao W

Mon Sep 28 00:00:00 EDT 2020

FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.

Cell death & disease

Zhou K,Chen H,Lin J,Xu H,Wu H,Bao G,Li J,Deng X,Shui X,Gao W,Ding J,Xiao J,Xu H

Mon Nov 18 00:00:00 EST 2019

TGF-β1 inhibits the autophagy of podocytes by activating mTORC1 in IgA nephropathy.

Experimental cell research

Mao X,Xu Z,Xu X,Zeng M,Zhao Z,Zhang Z,Ding X,Wu H

Sun Dec 01 00:00:00 EST 2019

Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.

American journal of translational research

Lin J,Lin R,Li S,Wu H,Ding J,Xiang G,Li S,Wang Y,Lin D,Gao W,Kong J,Xu H,Zhou K

Tue Sep 29 00:00:00 EDT 2020

Betulinic Acid Enhances the Viability of Random-Pattern Skin Flaps by Activating Autophagy.

Frontiers in pharmacology

Li J,Bao G,ALyafeai E,Ding J,Li S,Sheng S,Shen Z,Jia Z,Lin C,Zhang C,Lou Z,Xu H,Gao W,Zhou K

Thu Oct 01 00:00:00 EDT 2020

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway.

International journal of biological sciences

Wu H,Ding J,Li S,Lin J,Jiang R,Lin C,Dai L,Xie C,Lin D,Xu H,Gao W,Zhou K

Mon Jan 13 00:00:00 EST 2020

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

EMBO reports

Arasaki K,Nagashima H,Kurosawa Y,Kimura H,Nishida N,Dohmae N,Yamamoto A,Yanagi S,Wakana Y,Inoue H,Tagaya M

Wed Aug 01 00:00:00 EDT 2018

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

EMBO reports

Arasaki K,Nagashima H,Kurosawa Y,Kimura H,Nishida N,Dohmae N,Yamamoto A,Yanagi S,Wakana Y,Inoue H,Tagaya M

Wed Aug 01 00:00:00 EDT 2018

Salvianolic Acid B Promotes the Survival of Random-Pattern Skin Flaps in Rats by Inducing Autophagy.

Frontiers in pharmacology

Lin J,Lin R,Li S,Wu H,Ding J,Xiang G,Li S,Wang Y,Lin D,Gao W,Kong J,Xu H,Zhou K

Sat Nov 04 00:00:00 EDT 2023

Salvianolic Acid B Promotes the Survival of Random-Pattern Skin Flaps in Rats by Inducing Autophagy.

Frontiers in pharmacology

Lin J,Lin R,Li S,Wu H,Ding J,Xiang G,Li S,Wang Y,Lin D,Gao W,Kong J,Xu H,Zhou K

Sat Nov 04 00:00:00 EDT 2023

β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.

Cell death & disease

Liu J,Li QX,Wang XJ,Zhang C,Duan YQ,Wang ZY,Zhang Y,Yu X,Li NJ,Sun JP,Yi F

Thu Apr 07 00:00:00 EDT 2016

β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.

Cell death & disease

Liu J,Li QX,Wang XJ,Zhang C,Duan YQ,Wang ZY,Zhang Y,Yu X,Li NJ,Sun JP,Yi F

Thu Apr 07 00:00:00 EDT 2016

Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon.

Autophagy

Xu D,Zhang T,Xiao J,Zhu K,Wei R,Wu Z,Meng H,Li Y,Yuan J

Fri Apr 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: catalytic phosphatidylinositol 3-kinase 3; catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI-3K Vps34p; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3

View more View less

Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34

View more View less

UniProt ID: (Human) Q8NEB9, (Rat) O88763, (Mouse) Q6PF93

View more View less

Entrez Gene ID: (Human) 5289, (Rat) 65052, (Mouse) 225326

View more View less

Function(s)

protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor kinase

Process(es)

autophagosome assembly cytokinesis protein phosphorylation phosphatidylinositol biosynthetic process endosome organization macroautophagy protein processing regulation of cytokinesis toll-like receptor 9 signaling pathway phosphatidylinositol-3-phosphate biosynthetic process cellular response to glucose starvation response to leucine early endosome to late endosome transport phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of protein secretion endocytosis autophagy cell cycle endosomal transport phosphorylation pexophagy cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

VPS34 (C terminal) Polyclonal Antibody

VPS34 (C terminal) Antibody (12452-1-AP) in ICC/IF

Product Details

12452-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!