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Versican Antibody (MA5-45625) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Versican monoclonal antibody (Product # MA5-45625) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue)... View More
Product Details
MA5-45625
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N351/23
Immunogen
Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932079
Product Specific Information
1 µg/mL of MA5-45625 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects >350kDa.
This antibody was formerly sold as clone S351-23.
Target Information
Versican is a member of the family of large aggregating proteoglycans (also including aggrecan, brevican, and neurocan). The protein shows wide tissue distribution which includes fibrous, articular, and elastic cartilages, as well as nervous, epidermal, arterial, and loose connective tissues. Versican V1 has been shown to enhance cell proliferation, and also induce cell transformation and protect cells from apoptosis. This was demonstrated through its ability to downregulate the expression of proapoptotic Bad. Versican's existence as a extracellular matrix protein in human airways has also lead to the discovery of an inflammatory role in asthmatics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan 2 (versican); Chondroitin sulfate proteoglycan core protein 2; GHAP; Glial hyaluronate-binding protein; heart defect; Large fibroblast proteoglycan; PG-M; PG-M core protein; V1 Neo; Versican core protein; Versican core protein precursor (Large fibroblast proteoglycan) (Chondroitin sulfate proteoglycan core protein 2) (PG-M); versican proteoglycan; Versican V0; WGN 1
Gene Aliases: 5430420N07Rik; 9430051N09; CSPG2; DPEAAE; ERVR; GHAP; hdf; NG2; PG-M; PG-M(V0); PG-M(V1); VCAN; WGN; WGN1
UniProt ID: (Human) P13611, (Rat) Q9ERB4
Entrez Gene ID: (Human) 1462, (Mouse) 13003, (Rat) 114122
skeletal system development
osteoblast differentiation
cell adhesion
multicellular organism development
central nervous system development
cell recognition
extracellular matrix organization
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
heart development
glial cell migration
regulation of phosphoprotein phosphatase activity
response to wounding
axon regeneration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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