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Alomone Labs, LTD
Vesicular Acetylcholine Transporter (VAChT) Polyclonal Antibody
FIGURE: 1 / 2
Vesicular Acetylcholine Transporter (VAChT) Antibody (ACT-003-200UL) in IHC (F)
Expression of VAChT in rat medial septum and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:400), followed by goat- Anti-rabbit-AlexaFluor-488. A. VAChT staining (green) in rat medial septum is detected in neuronal soma (arrows). B. Staining of spinal cord sections reveals VAChT labeling i... View More
Product Details
ACT-003-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495-509 of rat VAChT, Cytoplasmic, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC12716; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
Gene Aliases: rVAT; Slc18a3; VAChT; VAT
UniProt ID: (Rat) Q62666
Entrez Gene ID: (Mouse) 20508, (Rat) 60422
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