Proteintech

Villin Polyclonal Antibody

Villin Antibody in Immunocytochemistry (ICC/IF)

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Villin Antibody (16488-1-AP) in ICC/IF

Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Villin Antibody in Flow Cytometry (Flow)

Villin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16488-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:8,000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Villin Fusion Protein Ag9610 (1-350 aa encoded by BC017303)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTKLSAQVK GSLNITTPGL QIWRIEAMQM VPVPSSTFGS FFDGDCYIIL AIHKTASSLS YDIHYWIGQD SSLDEQGAAA IYTTQMDDFL KGRAVQHREV QGNESEAFRG YFKQGLVIRK GGVASGMKHV ETNSYDVQRL LHVKGKRNVV AGEVEMSWKS FNRGDVFLLD LGKLIIQWNG PESTRMERLR GMTLAKEIRD QERGGRTYVG VVDGENELAS PKLMEVMNHV LGKRRELKAA VPDTVVEPAL KAALKLYHVS DSEGNLVVRE VATRPLTQDL LSHEDCYILD QGGLKIYVWK GKKANEQEKK GAMSHALNFI KAKQYPPSTQ VEVQNDGAES AVFQQLFQKW T (1-350 aa encoded by BC017303)

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Thu Feb 23 00:00:00 EST 2023

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.

PLoS pathogens

Yang QY,Yang YL,Tang YX,Qin P,Wang G,Xie JY,Chen SX,Ding C,Huang YW,Zhu SJ

Wed Jun 01 00:00:00 EDT 2022

The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.

Dose-response : a publication of International Hormesis Society

Liu D,Peng R,Chen Z,Yu H,Wang S,Dong S,Li W,Shao W,Dai J,Li F,Jiang Q,Sun W

Fri Jul 22 00:00:00 EDT 2022

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

Clinical and translational medicine

Liu Z,Chen Y,Niu B,Yin D,Feng F,Gu S,An Q,Xu J,An N,Zhang J,Yi J,Yin W,Qin X,Hu X

Mon Mar 01 00:00:00 EST 2021

Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.

Frontiers in cell and developmental biology

Wang S,Kai L,Zhu L,Xu B,Chen N,Valencak TG,Wang Y,Shan T

Fri Aug 13 00:00:00 EDT 2021

Survival and cellular heterogeneity of epithelium in cultured mouse and rat precision-cut intestinal slices.

Toxicology in vitro : an international journal published in association with BIBRA

Biel C,Bigaeva E,Hesse M,Bomers JJM,van Summeren K,Teunis MAT,Vaessen S,Ten Klooster JP,Olinga P

Tue Dec 01 00:00:00 EST 2020

Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.

Proceedings of the National Academy of Sciences of the United States of America

Corry J,Arora N,Good CA,Sadovsky Y,Coyne CB

Tue Aug 29 00:00:00 EDT 2017

Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.

Proceedings of the National Academy of Sciences of the United States of America

Corry J,Arora N,Good CA,Sadovsky Y,Coyne CB

Tue Aug 29 00:00:00 EDT 2017

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

International journal of molecular sciences

Yang W,Sun Z,Yang B,Wang Q

Mon Jul 31 00:00:00 EDT 2017

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

International journal of molecular sciences

Yang W,Sun Z,Yang B,Wang Q

Mon Jul 31 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: OTTHUMP00000207159; Villin-1

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Gene Aliases: D2S1471; VIL; VIL1

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UniProt ID: (Human) P09327, (Mouse) Q62468

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Entrez Gene ID: (Human) 7429, (Mouse) 22349, (Rat) 316521

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Function(s)

calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding lysophosphatidic acid binding identical protein binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process actin filament binding actin binding metal ion binding molecular_function non-motor actin binding protein

Process(es)

intestinal D-glucose absorption protein complex assembly apoptotic process epidermal growth factor receptor signaling pathway regulation of cell shape response to bacterium positive regulation of epithelial cell migration actin filament polymerization actin filament depolymerization positive regulation of cell migration positive regulation of actin filament depolymerization epithelial cell differentiation positive regulation of actin filament bundle assembly regulation of microvillus length cellular response to hepatocyte growth factor stimulus positive regulation of multicellular organism growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process actin nucleation actin filament severing barbed-end actin filament capping regulation of actin nucleation actin filament capping cytoplasmic actin-based contraction involved in cell motility regulation of wound healing cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane terminal web assembly regulation of lamellipodium morphogenesis positive regulation of lamellipodium morphogenesis cytoskeleton organization microvillus assembly biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Villin Polyclonal Antibody

Villin Antibody (16488-1-AP) in ICC/IF

Product Details

16488-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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