Invitrogen

Villin Monoclonal Antibody (CWWB1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 References

View all (53) Villin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

Villin Antibody (MA5-12227) in IHC (P)

Formalin-fixed, paraffin-embedded human kidney stained with Villin antibody using peroxidase-conjugate and AEC chromogen. Note brush border and cytoplasmic staining of epithelial cells in the proximal tubules.

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Product Details

MA5-12227

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CWWB1

Immunogen

Human Villin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980388

Product Specific Information

MA5-12227 targets Villin in IHC (P) applications and shows reactivity with Human samples.

The MA5-12227 immunogen is human Villin protein.

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NPC1L1-dependent transport of 27-alkyne cholesterol in intestinal epithelial cells.

American journal of physiology. Cell physiology

Ticho AL,Calzadilla N,Malhotra P,Lee H,Anbazhagan AN,Saksena S,Dudeja PK,Lee D,Gill RK,Alrefai WA

MA5-12227 was used in Immunohistochemistry to show that alkyne cholesterol acts as a substrate for NPC1L1 and may serve as a nonradioactive tracer to measure cholesterol absorption in both in vitro and in vivo models.

Sat May 01 00:00:00 EDT 2021

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture.

Nature medicine

Li X,Nadauld L,Ootani A,Corney DC,Pai RK,Gevaert O,Cantrell MA,Rack PG,Neal JT,Chan CW,Yeung T,Gong X,Yuan J,Wilhelmy J,Robine S,Attardi LD,Plevritis SK,Hung KE,Chen CZ,Ji HP,Kuo CJ

MA5-12227 was used in immunohistochemistry - paraffin section to study the oncogenic transformation in primary organoid culture of gastrointestinal tissues

Tue Jul 01 00:00:00 EDT 2014

Pulmonary enteric adenocarcinoma: a study of the clinicopathologic and molecular status of nine cases.

International journal of clinical and experimental pathology

Wang CX,Liu B,Wang YF,Zhang RS,Yu B,Lu ZF,Shi QL,Zhou XJ

MA5-12227 was used in immunohistochemistry to report on nine cases of pulmonary enteric adenocarcinoma

Fri Dec 19 00:00:00 EST 2014

Efficient differentiation of human embryonic stem cells to definitive endoderm.

Nature biotechnology

D'Amour KA,Agulnick AD,Eliazer S,Kelly OG,Kroon E,Baetge EE

MA5-12227 was used in immunohistochemistry - paraffin section to elucidate the differentiation of human embryonic stem cells to definitive endoderm efficiently

Thu Dec 01 00:00:00 EST 2005

Phenotype of columnar-lined esophagus in rats with esophagogastroduodenal anastomosis: similarity to human Barrett's esophagus.

Laboratory investigation; a journal of technical methods and pathology

Su Y,Chen X,Klein M,Fang M,Wang S,Yang CS,Goyal RK

MA5-12227 was used in Immunohistochemistry to describe the morphology and phenotypic features of columnar-lined oesophagus and oesophageal adenocarcinoma in the rat model and compares them with the corresponding lesions in human Barrett's esophagus.

Tue Jun 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: OTTHUMP00000207159; Villin-1

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Gene Aliases: D2S1471; VIL; VIL1

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UniProt ID: (Human) P09327, (Mouse) Q62468

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Entrez Gene ID: (Human) 7429, (Mouse) 22349

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Function(s)

calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding lysophosphatidic acid binding identical protein binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process actin filament binding actin binding metal ion binding non-motor actin binding protein

Process(es)

intestinal D-glucose absorption protein complex assembly apoptotic process epidermal growth factor receptor signaling pathway regulation of cell shape response to bacterium positive regulation of epithelial cell migration actin filament polymerization actin filament depolymerization positive regulation of cell migration positive regulation of actin filament depolymerization epithelial cell differentiation positive regulation of actin filament bundle assembly regulation of microvillus length cellular response to hepatocyte growth factor stimulus positive regulation of multicellular organism growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process actin nucleation actin filament severing barbed-end actin filament capping regulation of actin nucleation actin filament capping cytoplasmic actin-based contraction involved in cell motility regulation of wound healing cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane terminal web assembly regulation of lamellipodium morphogenesis positive regulation of lamellipodium morphogenesis cytoskeleton organization microvillus assembly

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Villin Monoclonal Antibody (CWWB1)

Villin Antibody (MA5-12227) in IHC (P)

Product Details

MA5-12227

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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