Invitrogen

Vimentin Polyclonal Antibody

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures

10 References

View all (201) Vimentin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Vimentin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Vimentin Antibody (PA1-10003) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Chicken Polyclonal Antibody (Product # PA1-10003) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Chicken IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

Vimentin Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-10003

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:5,000

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Full length recombinant human vimentin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2216267

Product Specific Information

PA1-10003 can be used to study stem cells and generally to reveal the intermediate filament cytoskeleton. The immunogen used to generate this antibody was full length recombinant human vimentin. The antibody works well on all mammals tested to date, and it was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation.

Scientific reports

Gonzalez Pisfil M,Nadelson I,Bergner B,Rottmeier S,Thomae AW,Dietzel S

PA1-10003 was used in Immunocytochemistry-immunoflourescence to provide evidence that eight color FLIM-STED with a single depletion laser would be possible if suitable fluorochromes were identified and we confirm that a fluorochrome may have different lifetimes depending on the molecules to which it is coupled.

Thu Aug 18 00:00:00 EDT 2022

Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation.

Scientific reports

Gonzalez Pisfil M,Nadelson I,Bergner B,Rottmeier S,Thomae AW,Dietzel S

Thu Aug 18 00:00:00 EDT 2022

Functional microvascularization of human myocardium in vitro.

Cell reports methods

King O,Cruz-Moreira D,Sayed A,Kermani F,Kit-Anan W,Sunyovszki I,Wang BX,Downing B,Fourre J,Hachim D,Randi AM,Stevens MM,Rasponi M,Terracciano CM

PA1-10003 was used in In vivo experiments to study static and perfused models of human myocardial-microvascular interaction using a functionally vascularized in vitro model of human myocardium with widespread potential applications in basic and translational research.

Mon Sep 19 00:00:00 EDT 2022

Chemical and mechanical activation of resident cardiac macrophages in the living myocardial slice ex vivo model.

Basic research in cardiology

Waleczek FJG,Sansonetti M,Xiao K,Jung M,Mitzka S,Dendorfer A,Weber N,Perbellini F,Thum T

PA1-10003 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to demonstrate the approach of LMS to understand load-triggered cardiac inflammation and, thus, identify potential translationally important therapeutic targets.

Wed Nov 30 00:00:00 EST 2022

Cardiac Fibroblasts Mediate a Sexually Dimorphic Fibrotic Response to β-Adrenergic Stimulation.

Journal of the American Heart Association

Peter AK,Walker CJ,Ceccato T,Trexler CL,Ozeroff CD,Lugo KR,Perry AR,Anseth KS,Leinwand LA

PA1-10003 was used in Immunocytochemistry to explore the contribution of cardiac fibroblasts to the sexual dimorphism seen in cardiovascular disease.

Tue Jun 01 00:00:00 EDT 2021

Cardiac Fibroblasts Mediate a Sexually Dimorphic Fibrotic Response to β-Adrenergic Stimulation.

Journal of the American Heart Association

Peter AK,Walker CJ,Ceccato T,Trexler CL,Ozeroff CD,Lugo KR,Perry AR,Anseth KS,Leinwand LA

PA1-10003 was used in Immunocytochemistry to explore the contribution of cardiac fibroblasts to the sexual dimorphism seen in cardiovascular disease.

Tue Jun 01 00:00:00 EDT 2021

Astrocytes Exhibit a Protective Role in Neuronal Firing Patterns under Chemically Induced Seizures in Neuron-Astrocyte Co-Cultures.

International journal of molecular sciences

Ahtiainen A,Genocchi B,Tanskanen JMA,Barros MT,Hyttinen JAK,Lenk K

PA1-10003 was used in Immunocytochemistry-immunofluorescence to assess the modulatory roles of astrocytes during epileptic-like conditions and in compensating drug-elicited hyperactivity.

Thu Nov 25 00:00:00 EST 2021

FDPS promotes glioma growth and macrophage recruitment by regulating CCL20 via Wnt/β-catenin signalling pathway.

Journal of cellular and molecular medicine

Chen Z,Chen G,Zhao H

PA1-10003 was used in Western Blotting to show that enhancement in FDPS level is observed in glioma tissues and associate with poor prognosis, contributed to tumour growth.

Sat Aug 01 00:00:00 EDT 2020

Defining the Cardiac Fibroblast Secretome in a Fibrotic Microenvironment.

Journal of the American Heart Association

Ceccato TL,Starbuck RB,Hall JK,Walker CJ,Brown TE,Killgore JP,Anseth KS,Leinwand LA

PA1-10003 was used in Immunohistochemistry to determine how the cardiac fibroblasts secretome changes with matrix stiffness and biochemical cues and how this affects cardiac myocytes via paracrine signaling.

Tue Oct 20 00:00:00 EDT 2020

Tanycytes and a differential fatty acid metabolism in the hypothalamus.

Glia

Hofmann K,Lamberz C,Piotrowitz K,Offermann N,But D,Scheller A,Al-Amoudi A,Kuerschner L

PA110003 was used in immunohistochemistry to report that saturated and unsaturated fatty acids are differentially processed in the murine hypothalamus

Wed Feb 01 00:00:00 EST 2017

Identification of interstitial Cajal-like cells in the human thoracic duct.

Cells, tissues, organs

Briggs Boedtkjer D,Rumessen J,Baandrup U,Skov Mikkelsen M,Telinius N,Pilegaard H,Aalkjaer C,Hjortdal V

PA1-10003 was used in immunohistochemistry to report on the presence of interstitial Cajal-like cells in the human thoracic duct

Fri Sep 20 00:00:00 EDT 2013

Proteomic analysis reveals virus-specific Hsp25 modulation in cardiac myocytes.

Journal of proteome research

Li L,Sevinsky JR,Rowland MD,Bundy JL,Stephenson JL,Sherry B

Published figure using Vimentin polyclonal antibody (Product # PA1-10003) in Immunofluorescence

Fri May 07 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; intermediate filament protein; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Mouse) P20152, (Rat) P31000

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Entrez Gene ID: (Pig) 100522394, (Bovine) 280955, (Chicken) 420519, (Human) 7431, (Horse) 100056088, (Mouse) 22352, (Rat) 81818

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Function(s)

double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein domain specific binding identical protein binding scaffold protein binding keratin filament binding protein binding glycoprotein binding protein C-terminus binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

negative regulation of neuron projection development astrocyte development positive regulation of collagen biosynthetic process regulation of mRNA stability intermediate filament organization positive regulation of translation Bergmann glial cell differentiation SMAD protein signal transduction lens fiber cell development cellular response to lipopolysaccharide cellular response to muramyl dipeptide cellular response to interferon-gamma positive regulation of gene expression intermediate filament-based process regulation of axonogenesis positive regulation of glial cell proliferation regulation of Schwann cell migration in utero embryonic development positive regulation of superoxide anion generation movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization

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Vimentin Polyclonal Antibody

Vimentin Antibody (PA1-10003) in ICC/IF

Product Details

PA1-10003

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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