Invitrogen

Vimentin Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

9 References

View all (201) Vimentin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (PA1-16759) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Chicken Polyclonal Antibody (Product # PA1-16759) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Chicken IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

Vimentin Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-16759

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:20,000

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:200-1:5000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Horse, Human, Mouse, Pig, Rat

Published species

Human, Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

The immunogen used to generate our antibody was recombinant vimentin purified from E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

16.6 mg/mL

Purification

IgY fraction

Storage buffer

PBS

Contains

0.035% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2257294

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Systematic estimation of biological age of in vitro cell culture systems by an age-associated marker panel.

Frontiers in aging

Hartmann C,Herling L,Hartmann A,Köckritz V,Fuellen G,Walter M,Hermann A

PA1-16759 was used in Immunohistochemistry-immunofluorescence to suggest an easy-to-use biomarker-based panel of classical age markers to estimate the biological age of cell culture systems that can be used in standard cell culture laboratories.

Tue Mar 07 00:00:00 EST 2023

RhoA drives actin compaction to restrict axon regeneration and astrocyte reactivity after CNS injury.

Neuron

Stern S,Hilton BJ,Burnside ER,Dupraz S,Handley EE,Gonyer JM,Brakebusch C,Bradke F

PA1-16759 was used in Immunohistochemistry to demonstrate how extracellular inhibitors regulate axon regeneration, shed light on the capacity of reactive astrocytes to be growth inhibitory after CNS injury, and reveal cell-specific RhoA targeting as a promising therapeutic avenue.

Wed Nov 03 00:00:00 EDT 2021

ROCK2 inhibition triggers the collective invasion of colorectal adenocarcinomas.

The EMBO journal

Libanje F,Raingeaud J,Luan R,Thomas Z,Zajac O,Veiga J,Marisa L,Adam J,Boige V,Malka D,Goéré D,Hall A,Soazec JY,Prall F,Gelli M,Dartigues P,Jaulin F

PA1-16759 was used in Immunohistochemistry-immunofluorescence to identify FARP2 as the Rac1 GEF necessary for CRC collective invasion.

Mon Jul 15 00:00:00 EDT 2019

Biomimetic electromechanical stimulation to maintain adult myocardial slices in vitro.

Nature communications

Watson SA,Duff J,Bardi I,Zabielska M,Atanur SS,Jabbour RJ,Simon A,Tomas A,Smolenski RT,Harding SE,Perbellini F,Terracciano CM

PA1-16759 was used in Immunohistochemistry-immunofluorescence to show biomimetic electromechanical stimulation of adult cardiac tissue can maintain structural, functional and transcriptional properties.

Wed May 15 00:00:00 EDT 2019

4-methylumbelliferone Prevents Liver Fibrosis by Affecting Hyaluronan Deposition, FSTL1 Expression and Cell Localization.

International journal of molecular sciences

Andreichenko IN,Tsitrina AA,Fokin AV,Gabdulkhakova AI,Maltsev DI,Perelman GS,Bulgakova EV,Kulikov AM,Mikaelyan AS,Kotelevtsev YV

PA1-16759 was used in Immunohistochemistry to provide insight in the alleviation of CCl4 induced liver fibrosis by 4-methylumbelliferone through the reduction of hyaluronan deposition, dowregulation of FSTL1 expression and suppression of hepatic stellate cell trans-differentiation and altered macrophage localisation.

Fri Dec 13 00:00:00 EST 2019

The LepR-mediated leptin transport across brain barriers controls food reward.

Molecular metabolism

Di Spiezio A,Sandin ES,Dore R,Müller-Fielitz H,Storck SE,Bernau M,Mier W,Oster H,Jöhren O,Pietrzik CU,Lehnert H,Schwaninger M

Published figure using Vimentin polyclonal antibody (Product # PA1-16759) in Immunofluorescence

Thu Feb 01 00:00:00 EST 2018

NF-κB activation is cell type-specific in the heart.

Virology

Rivera-Serrano EE,Sherry B

Published figure using Vimentin polyclonal antibody (Product # PA1-16759) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2017

NF-κB activation is cell type-specific in the heart.

Virology

Rivera-Serrano EE,Sherry B

Published figure using Vimentin polyclonal antibody (Product # PA1-16759) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2017

Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis.

Nature communications

Müller-Fielitz H,Stahr M,Bernau M,Richter M,Abele S,Krajka V,Benzin A,Wenzel J,Kalies K,Mittag J,Heuer H,Offermanns S,Schwaninger M

Published figure using Vimentin polyclonal antibody (Product # PA1-16759) in Immunofluorescence

Thu Sep 07 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; intermediate filament protein; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3, (Mouse) P20152, (Rat) P31000

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Entrez Gene ID: (Pig) 100522394, (Bovine) 280955, (Chicken) 420519, (Human) 7431, (Horse) 100056088, (Dog) 477991, (Mouse) 22352, (Rat) 81818

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Function(s)

double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein domain specific binding identical protein binding scaffold protein binding keratin filament binding protein binding glycoprotein binding protein C-terminus binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

negative regulation of neuron projection development astrocyte development positive regulation of collagen biosynthetic process regulation of mRNA stability intermediate filament organization positive regulation of translation Bergmann glial cell differentiation SMAD protein signal transduction lens fiber cell development cellular response to lipopolysaccharide cellular response to muramyl dipeptide cellular response to interferon-gamma positive regulation of gene expression intermediate filament-based process regulation of axonogenesis positive regulation of glial cell proliferation regulation of Schwann cell migration in utero embryonic development positive regulation of superoxide anion generation movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization

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Vimentin Polyclonal Antibody

Vimentin Antibody (PA1-16759) in ICC/IF

Product Details

PA1-16759

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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