Invitrogen

Vimentin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

21 Published Figures

28 References

View all (201) Vimentin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Vimentin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 48

Vimentin Antibody (PA5-27231) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70 confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Vimentin Polyclonal Antibody (Product # PA5-27231) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Immunohistochemistry (IHC)

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation (ICC/IF, IHC, IP)

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Product Details

PA5-27231

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 8 publications 8 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

1:100-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 61 and 426 of Human Vimentin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544707

Product Specific Information

Recommended positive controls: 293T, HeLa, MDA-MB-231, U87-MG, SK-N-SH, IMR32, SK-N-AS, NIH-3T3, A549.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (85%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191.

Experimental and therapeutic medicine

Xu X,Cai B,Liu Y,Liu R,Li J

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Wed Mar 01 00:00:00 EST 2023

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191.

Experimental and therapeutic medicine

Xu X,Cai B,Liu Y,Liu R,Li J

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Wed Mar 01 00:00:00 EST 2023

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Haematologica

Michalik S,Siegerist F,Palankar R,Franzke K,Schindler M,Reder A,Seifert U,Cammann C,Wesche J,Steil L,Hentschker C,Gesell-Salazar M,Reisinger E,Beer M,Endlich N,Greinacher A,Völker U

PA5-27231 was used in Immunocytochemistry to conclude that these differences in impurities together with EDTAinduced capillary leakage might contribute to the higher incidence rate of VITT associated with ChAdOx1 nCoV-19 compared to Ad26.COV2.S.

Fri Apr 01 00:00:00 EDT 2022

Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.

International journal of molecular sciences

Camino T,Lago-Baameiro N,Sueiro A,Bravo SB,Couto I,Santos FF,Baltar J,Casanueva FF,Pardo M

PA5-27231 was used in Western Blotting to isolate batosomes from lean and obese Sprague-Dawley rats, and to establish reference proteome maps.

Fri Sep 16 00:00:00 EDT 2022

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-27231 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 EDT 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-27231 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 EDT 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

PA5-27231 was used in Immunohistochemistry-immunofluorescence to demonstrate that normally quiescent lens epithelial cells can be reactivated for epithelialization repair in response to UV-induced damage.

Wed Dec 01 00:00:00 EST 2021

Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model.

Mediators of inflammation

Arafat EA,Marzouk RE,Mostafa SA,Hamed WHE

PA5-27231 was used in Immunohistochemistry to examine the effect of nanocurcumin (NC) on the morphometric and immunohistochemical characterization of telocytes in the ulcerative colitis (UC) rat model.

Mon Jan 24 00:00:00 EST 2022

Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model.

Mediators of inflammation

Arafat EA,Marzouk RE,Mostafa SA,Hamed WHE

Mon Jan 24 00:00:00 EST 2022

Establishment and Characterization of a Novel Dedifferentiated Chondrosarcoma Cell Line DDCS2.

Cancer control : journal of the Moffitt Cancer Center

Li X,Dean DC,Ferreira A,Nelson SD,Hornicek FJ,Yu S,Duan Z

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Fri Jan 21 00:00:00 EST 2022

Establishment and Characterization of a Novel Dedifferentiated Chondrosarcoma Cell Line DDCS2.

Cancer control : journal of the Moffitt Cancer Center

Li X,Dean DC,Ferreira A,Nelson SD,Hornicek FJ,Yu S,Duan Z

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Fri Jan 21 00:00:00 EST 2022

D-Propranolol Impairs EGFR Trafficking and Destabilizes Mutant p53 Counteracting AKT Signaling and Tumor Malignancy.

Cancers

Barra J,Cerda-Infante J,Sandoval L,Gajardo-Meneses P,Henriquez JF,Labarca M,Metz C,Venegas J,Retamal C,Oyanadel C,Cancino J,Soza A,Cuello MA,Roa JC,Montecinos VP,Gonzalez A

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Tue Jul 20 00:00:00 EDT 2021

Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.

Journal of orthopaedic surgery and research

Wang T,Zhang C,Wang S

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Mon Dec 20 00:00:00 EST 2021

E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways.

Genetic testing and molecular biomarkers

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blot to conclude that E2F5 promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways.

Mon Mar 01 00:00:00 EST 2021

Differential expression and inhibitory effects of aquaporins on the development of adenomyosis.

Molecular medicine reports

Jia L,Liu Y,Han Y,Zhou X,Wang F

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Sun Nov 01 00:00:00 EDT 2020

Differential expression and inhibitory effects of aquaporins on the development of adenomyosis.

Molecular medicine reports

Jia L,Liu Y,Han Y,Zhou X,Wang F

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Sun Nov 01 00:00:00 EDT 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 EDT 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 EDT 2020

Periconceptional alcohol exposure causes female-specific perturbations to trophoblast differentiation and placental formation in the rat.

Development (Cambridge, England)

Kalisch-Smith JI,Steane SE,Simmons DG,Pantaleon M,Anderson ST,Akison LK,Wlodek ME,Moritz KM

PA5-27231 was used in Immunohistochemistry to show periconceptional alcohol exposure causes reduced placental volume at embryonic day 15 due to affecting trophoblast differentiation, embryo-uterine communication, and formation of the placental vasculature.

Mon Jun 10 00:00:00 EDT 2019

Citrullination Controls Dendritic Cell Transdifferentiation into Osteoclasts.

Journal of immunology (Baltimore, Md. : 1950)

Krishnamurthy A,Ytterberg AJ,Sun M,Sakuraba K,Steen J,Joshua V,Tarasova NK,Malmström V,Wähämaa H,Réthi B,Catrina AI

PA5-27231 was used in Immunocytochemistry-immunoflourescence to investigate whether protein citrullination and RA-specific anti-citrullinated protein could regulate human blood-derived DC-OC transdifferentiation.

Sat Jun 01 00:00:00 EDT 2019

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

PA5-27231 was used in Immunoprecipitation to study the molecular mechanism of Sirtuin in hepatocellular carcinoma migration.

Wed Sep 30 00:00:00 EDT 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

PA5-27231 was used in Immunoprecipitation to study the molecular mechanism of Sirtuin in hepatocellular carcinoma migration.

Wed Sep 30 00:00:00 EDT 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

PA5-27231 was used in Immunoprecipitation to study the molecular mechanism of Sirtuin in hepatocellular carcinoma migration.

Wed Sep 30 00:00:00 EDT 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

PA5-27231 was used in Immunoprecipitation to study the molecular mechanism of Sirtuin in hepatocellular carcinoma migration.

Wed Sep 30 00:00:00 EDT 2020

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

Cell biology international

Zhang Y,Zhang Y

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Sun Jul 01 00:00:00 EDT 2018

Myostatin promotes the epithelial-to-mesenchymal transition of the dermomyotome during somitogenesis.

Developmental dynamics : an official publication of the American Association of Anatomists

Zhou Y,Zhang Y,Zhu D

PA5-27231 was used in Immunohistochemistry (Frozen) to suggest that MSTN regulates myogenesis by promoting EMT during somitogenesis and provide novel insights into the functions of MSTN during early embryonic myogenesis.

Sat Dec 01 00:00:00 EST 2018

Molecular oncology

Kryza T,Silva LM,Bock N,Fuhrman-Luck RA,Stephens CR,Gao J,Samaratunga H,Lawrence MG,Hooper JD,Dong Y,Risbridger GP,Clements JA

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Sun Oct 01 00:00:00 EDT 2017

Cytosolic Hsp90α and its mitochondrial isoform Trap1 are differentially required in a breast cancer model.

Oncotarget

Vartholomaiou E,Madon-Simon M,Hagmann S,Mühlebach G,Wurst W,Floss T,Picard D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Tue Mar 14 00:00:00 EDT 2017

Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.

Future oncology (London, England)

Fabbri R,Macciocca M,Vicenti R,Paradisi R,Klinger FG,Pasquinelli G,Spisni E,Seracchioli R,Papi A

PA5-27231 was used in immunocytochemistry to analyze the induction of apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue by cisplatin and doxorubicin

Fri Jul 01 00:00:00 EDT 2016

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2016

Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.

PloS one

Kolahi K,Louey S,Varlamov O,Thornburg K

PA5-27231 was used in Immunohistochemistry to suggest a new role for cytotrophoblast in regulating placental fatty acid uptake and metabolism.

Tue Feb 21 00:00:00 EST 2017

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Human) P08670, (Mouse) P20152, (Rat) P31000

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Entrez Gene ID: (Human) 7431, (Mouse) 22352, (Rat) 81818

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Function(s)

glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

positive regulation of gene expression negative regulation of neuron projection development astrocyte development intermediate filament-based process intermediate filament organization regulation of axonogenesis Bergmann glial cell differentiation positive regulation of glial cell proliferation SMAD protein signal transduction lens fiber cell development regulation of Schwann cell migration movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization

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Vimentin Polyclonal Antibody

Vimentin Antibody (PA5-27231) in ICC/IF

Product Details

PA5-27231

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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