Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Vimentin Monoclonal Antibody (V9), Alexa Fluor™ 647
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Vimentin Antibody (MA5-11883-A647) in IHC (P)
Immunohistochemical analysis of Vimentin was performed using formalin-fixed paraffin-embedded human breast infiltrating ductal carcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 d... View More
Product Details
MA5-11883-A647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Horse,
Cat,
Gerbil,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
V9
Immunogen
Purified vimentin from pig eye lens
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2662891
Product Specific Information
The MA511883A647 immunogen is purified vimentin from pig eye lens.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; intermediate filament protein; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus
Gene Aliases: CTRCT30; HEL113; VIM
UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3, (Mouse) P20152, (Rat) P31000
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein domain specific binding
identical protein binding
scaffold protein binding
keratin filament binding
protein binding
glycoprotein binding
protein C-terminus binding
structural molecule activity
kinase binding
protein kinase binding
protein phosphatase 2A binding
negative regulation of neuron projection development
astrocyte development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
intermediate filament organization
positive regulation of translation
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
positive regulation of gene expression
intermediate filament-based process
regulation of axonogenesis
positive regulation of glial cell proliferation
regulation of Schwann cell migration
in utero embryonic development
positive regulation of superoxide anion generation
movement of cell or subcellular component
viral process
muscle filament sliding
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
fat cell differentiation
decidualization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support