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Invitrogen
Vimentin Monoclonal Antibody (VI-10)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
Vimentin Antibody (MA1-10459) in ICC/IF
Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Mouse Monoclonal Antibody (Product # MA1-10459) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ... View More
Product Details
MA1-10459
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
VI-10
Immunogen
not available
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_11154471
Product Specific Information
This antibody reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; intermediate filament protein; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus
Gene Aliases: CTRCT30; HEL113; VIM
UniProt ID: (Pig) P02543, (Human) P08670, (Mouse) P20152, (Rat) P31000
Entrez Gene ID: (Pig) 100522394, (Chicken) 420519, (Human) 7431, (Mouse) 22352, (Rat) 81818
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein domain specific binding
identical protein binding
scaffold protein binding
keratin filament binding
glycoprotein binding
protein binding
protein C-terminus binding
structural molecule activity
kinase binding
protein kinase binding
protein phosphatase 2A binding
negative regulation of neuron projection development
astrocyte development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
intermediate filament organization
positive regulation of translation
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
positive regulation of gene expression
intermediate filament-based process
regulation of axonogenesis
positive regulation of glial cell proliferation
regulation of Schwann cell migration
movement of cell or subcellular component
viral process
muscle filament sliding
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
fat cell differentiation
decidualization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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