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Vinculin Antibody (PA5-14325) in IHC (P)
Immunohistochemistry analysis of Vinculin in formalin-fixed and paraffin-embedded human skeletal muscle tissue. Samples were incubated with Vinculin polyclonal antibody (Product # PA5-14325) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-14325
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human VINC
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2214492
Product Specific Information
This antibody is predicted to react with chicken, porcine and rat based on sequence homology.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 114; Metavinculin; metavinculin {ECO:0000250|UniProtKB:P18206}; MV; RP11-178G16.3; vcl {ECO:0000250|UniProtKB:P18206}; vinc; Vinculin; vinculin {ECO:0000250|UniProtKB:P18206}
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL
UniProt ID: (Human) P18206, (Mouse) Q64727, (Rat) P85972
Entrez Gene ID: (Human) 7414, (Mouse) 22330, (Rat) 305679
morphogenesis of an epithelium
cell adhesion
cell-matrix adhesion
lamellipodium assembly
regulation of cell migration
adherens junction assembly
protein localization to cell surface
apical junction assembly
axon extension
platelet aggregation
epithelial cell-cell adhesion
platelet degranulation
movement of cell or subcellular component
muscle contraction
negative regulation of cell migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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