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WIPI1 Antibody (PA5-110581) in WB
Western blot analysis of WIPI1 in extracts of various cell lines. Samples were incubated with WIPI1 Polyclonal antibody (Product # PA5-110581) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-110581
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 187-446 of human WIPI1 (NP_060453.3).
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855992
Product Specific Information
Immunogen sequence: GTLAAITFNA SGSKLASASE KGTVIRVFSV PDGQKLYEFR RGMKRYVTIS SLVFSMDSQF LCASSNTETV HIFKLEQVTN SRPEEPSTWS GYMGKMFMAA TNYLPTQVSD MMHQDRAFAT ARLNFSGQRN ICTLSTIQKL PRLLVASSSG HLYMYNLDPQ DGGECVLIKT HSLLGSGTTE ENKENDLRPS LPQSYAATVA RPSASSASTV PGYSEDGGAL RGEVIPEHEF ATGPVCLDDE NEFPPIILCR GNQKGKTKQS
Target Information
WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Atg18 protein homolog; WD repeat domain phosphoinositide-interacting protein 1; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WD40 repeat protein Interacting with phosphoInositides of 49kDa; WIPI 49 kDa; WIPI-1; WIPI-1 alpha
Gene Aliases: 4930533H01Rik; ATG18; ATG18A; AW411817; D11Ertd498e; RGD1307754; WIPI1; WIPI49
UniProt ID: (Human) Q5MNZ9, (Mouse) Q8R3E3
Entrez Gene ID: (Human) 55062, (Rat) 303630, (Mouse) 52639
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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