Proteintech

WNT1 Polyclonal Antibody

FIGURE: 1 / 1

WNT1 Antibody (27935-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Product Details

27935-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WNT1 Fusion Protein Ag27380

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

300 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prolonged exposure to the herbicide atrazine promotes kidney fibrosis by activating Wnt/β-catenin signaling in rats.

Environmental toxicology

Wang T,Huang X,Liu J,Liu W,Yang Z,He K,Chen J,Zhao L

Mon May 01 00:00:00 EDT 2023

Prolonged exposure to the herbicide atrazine promotes kidney fibrosis by activating Wnt/β-catenin signaling in rats.

Environmental toxicology

Wang T,Huang X,Liu J,Liu W,Yang Z,He K,Chen J,Zhao L

Mon May 01 00:00:00 EDT 2023

Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke.

Neural regeneration research

Wang J,He P,Tian Q,Luo Y,He Y,Liu C,Gong P,Guo Y,Ye Q,Li M

Fri Sep 01 00:00:00 EDT 2023

Genotypic and Phenotypic Spectrum and Pathogenesis of WNT1 Variants in a Large Cohort of Patients With OI/Osteoporosis.

The Journal of clinical endocrinology and metabolism

Hu J,Lin X,Gao P,Zhang Q,Zhou B,Wang O,Jiang Y,Xia W,Xing X,Li M

Fri Jun 16 00:00:00 EDT 2023

Hepatoprotective effect of protocatechuic acid against type 2 diabetes-induced liver injury.

Pharmaceutical biology

Xu K,Lu G,Feng Q,Chen S,Wang Y

Fri Dec 01 00:00:00 EST 2023

QiHuangYiShen Granules Modulate the Expression of LncRNA MALAT1 and Attenuate Epithelial-Mesenchymal Transition in Kidney of Diabetic Nephropathy Rats.

Evidence-based complementary and alternative medicine : eCAM

Yuan LS,Du H,Ren QY,Yang RL,Liu SW,Liu SY,Shi KF,Wang B,Meng XF,Li TX,Zhang N

Sat Feb 11 00:00:00 EST 2023

The protective effect of lncRNA NEAT1/miR-122-5p/Wnt1 axis on hippocampal damage in hepatic ischemic reperfusion young mice.

Cellular signalling

Dong Z,Jia L,Han W,Wang Y,Sheng M,Ren Y,Weng Y,Li H,Yu W

Sat Jul 01 00:00:00 EDT 2023

LncRNA RPPH1 acts as a molecular sponge for miR-122 to regulate Wnt1/β-catenin signaling in hepatocellular carcinoma.

International journal of medical sciences

Zhou J,Shi K,Huang W,Zhang Y,Chen Q,Mou T,Wu Z,Wei X

Wed Jan 11 00:00:00 EST 2023

FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells.

Medical oncology (Northwood, London, England)

Yang Y,Li Y,Yang Q,Liu Z,Chang X,Yang H,Liu J,Li Z,Zuo D

Wed Dec 28 00:00:00 EST 2022

SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer.

iScience

Bhattacharya A,Wei J,Song W,Gao B,Tian C,Wu SA,Wang J,Chen L,Fang D,Qi L

Fri Oct 21 00:00:00 EDT 2022

Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells.

Frontiers in pharmacology

Yu PC,Liu D,Han ZX,Liang F,Hao CY,Lei YT,Guo CR,Wang WH,Li XH,Yang XN,Li CZ,Yu Y,Fan YZ

Sat Mar 05 00:00:00 EST 2022

Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats.

Microvascular research

Qin X,Qin H,Li Z,Xue S,Huang B,Liu X,Wang D

Sat Jan 01 00:00:00 EST 2022

Kangxianruangan granule‑containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt‑1/β‑catenin signaling pathway.

Molecular medicine reports

Tang W,Xue J,Luo L,Wang Y,Cai X,Liu Y,Huang D,Wang X,He T,Lu D,Yang F

Tue Feb 01 00:00:00 EST 2022

Kangxianruangan granule‑containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt‑1/β‑catenin signaling pathway.

Molecular medicine reports

Tang W,Xue J,Luo L,Wang Y,Cai X,Liu Y,Huang D,Wang X,He T,Lu D,Yang F

Tue Feb 01 00:00:00 EST 2022

SEC14L3 plays a tumor-suppressive role in breast cancer through a Wnt/β-catenin-related way.

Experimental cell research

Zhu Q,Wan NB,Deng HW,Lu LL,Zhang Y,He X,Liu H,He Y

Mon Aug 01 00:00:00 EDT 2022

Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.

Frontiers in oncology

Wang H,Zhao S,Liu Y,Sun F,Huang X,Wu T

Sat Jul 16 00:00:00 EDT 2022

Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.

Frontiers in oncology

Wang H,Zhao S,Liu Y,Sun F,Huang X,Wu T

Sat Jul 16 00:00:00 EDT 2022

A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis.

Cell death & disease

Wang D,Chen J,Li B,Jiang Q,Liu L,Xia Z,Zheng Q,Li M,Li D

Thu Apr 07 00:00:00 EDT 2022

MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1).

Bioengineered

Song GL,Xiao M,Wan XY,Deng J,Ling JD,Tian YG,Li M,Yin J,Zheng RY,Tang Y,Liu GY

Wed Dec 01 00:00:00 EST 2021

Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.

Microbial biotechnology

Zhao X,Li S,Ding J,Wei J,Tian P,Wei H,Chen T

Wed Sep 01 00:00:00 EDT 2021

ODAM promotes junctional epithelium-related gene expression via activation of WNT1 signaling pathway in an ameloblast-like cell line ALC.

Journal of periodontal research

Song D,Yang S,Tan T,Wang R,Ma Z,Wang Y,Wang L

Tue Jun 01 00:00:00 EDT 2021

ODAM promotes junctional epithelium-related gene expression via activation of WNT1 signaling pathway in an ameloblast-like cell line ALC.

Journal of periodontal research

Song D,Yang S,Tan T,Wang R,Ma Z,Wang Y,Wang L

Tue Jun 01 00:00:00 EDT 2021

Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.

Molecular medicine reports

Zhou N,Yan B,Ma J,Jiang H,Li L,Tang H,Ji F,Yao Z

Wed Sep 01 00:00:00 EDT 2021

miR‑483‑3p promotes the osteogenesis of human osteoblasts by targeting Dikkopf 2 (DKK2) and the Wnt signaling pathway.

International journal of molecular medicine

Zhou B,Peng K,Wang G,Chen W,Liu P,Chen F,Kang Y

Thu Oct 01 00:00:00 EDT 2020

Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling.

Cancer management and research

Ping MH

Mon Apr 18 00:00:00 EDT 2022

Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling.

Cancer management and research

Ping MH

Mon Apr 18 00:00:00 EDT 2022

MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/β-catenin signaling pathway.

European review for medical and pharmacological sciences

Song J,Zhu XM,Wei QY

Sun Nov 01 00:00:00 EDT 2020

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Aging

Sha L,Ma D,Chen C

Sun Dec 13 00:00:00 EST 2020

Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation.

Journal of ethnopharmacology

Wu F,Shao Q,Hu M,Zhao Y,Dong R,Fang K,Xu L,Zou X,Lu F,Li J,Chen G

Fri Apr 24 00:00:00 EDT 2020

Exercise Promotes the Osteoinduction of HA/β-TCP Biomaterials via the Wnt Signaling Pathway.

Metabolites

Cheng L,Khalaf AT,Lin T,Ran L,Shi Z,Wan J,Zhou X,Zou L

Thu Mar 05 00:00:00 EST 2020

Agmatine Attenuates Liver Ischemia Reperfusion Injury by Activating Wnt/β-catenin Signaling in Mice.

Transplantation

Han Z,Li Y,Yang B,Tan R,Wang M,Zhang B,Dai C,Wei L,Chen D,Chen Z

Tue Sep 01 00:00:00 EDT 2020

RFC3 induces epithelial‑mesenchymal transition in lung adenocarcinoma cells through the Wnt/β‑catenin pathway and possesses prognostic value in lung adenocarcinoma.

International journal of molecular medicine

Gong S,Qu X,Yang S,Zhou S,Li P,Zhang Q

Sun Dec 01 00:00:00 EST 2019

Bioinformatics

Protein Aliases: Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1; WNT1

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UniProt ID: (Human) P04628, (Mouse) P04426

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Entrez Gene ID: (Human) 7471, (Mouse) 22408

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Function(s)

frizzled binding cytokine activity morphogen activity protein domain specific binding transcription regulatory region DNA binding receptor agonist activity receptor binding protein binding intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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WNT1 Polyclonal Antibody

WNT1 Antibody (27935-1-AP) in WB

Product Details

27935-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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