Invitrogen

WNT1 Polyclonal Antibody

View all (14) WNT1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-34776

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 234 of WNT1 (Uniprot ID#P04628)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552128

Product Specific Information

Recommended positive controls: 30 µg 293T whole cell lysate/extract, 30 µg whole cell lysate/extract of human WNT1-transfected 293T cells.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (81%), Bovine (99%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; INT1; OI15; WNT1

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UniProt ID: (Human) P04628

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Entrez Gene ID: (Human) 7471, (Rat) 24881

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Function(s)
frizzled binding cytokine activity morphogen activity protein domain specific binding transcription regulatory region DNA binding receptor agonist activity molecular_function intercellular signal molecule
Process(es)
embryonic axis specification branching involved in ureteric bud morphogenesis positive regulation of protein phosphorylation cell-cell signaling myoblast fusion positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion Wnt signaling pathway spinal cord association neuron differentiation diencephalon development central nervous system morphogenesis cerebellum formation forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development negative regulation of cell-cell adhesion negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway ubiquitin-dependent SMAD protein catabolic process midbrain development organ regeneration T cell differentiation in thymus cell proliferation in midbrain inner ear morphogenesis signal transduction in response to DNA damage positive regulation of insulin-like growth factor receptor signaling pathway canonical Wnt signaling pathway involved in negative regulation of apoptotic process cell fate commitment negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation neuron fate determination neuron development positive regulation of sequence-specific DNA binding transcription factor activity Spemann organizer formation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway bone development positive regulation of dermatome development hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation positive regulation of canonical Wnt signaling pathway negative regulation of cell aging beta-catenin destruction complex disassembly Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process myotube differentiation metencephalon development neuron differentiation midbrain-hindbrain boundary development negative regulation of cell differentiation neuron fate commitment
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