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WNT11 Antibody (PA5-47362) in ICC/IF
Immunocytochemistry analysis of WNT11 in immersion fixed LNCaP human prostate cancer cell line. Samples were incubated in WNT11 polyclonal antibody (Product # PA5-47362) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47362
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse Wnt-11 peptide Leu39-Ala79 and Lys225-Arg297
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609853
Product Specific Information
In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) Wnt-3A is observed and less than 1% cross-reactivity with rmWnt-5A is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-11; wingless-related MMTV integration site 11; wingless-type MMTV integration site family, member 11
Gene Aliases: HWNT11; Wnt-11; WNT11
UniProt ID: (Human) O96014, (Mouse) P48615
Entrez Gene ID: (Human) 7481, (Mouse) 22411
osteoblast differentiation
epithelial to mesenchymal transition
outflow tract morphogenesis
planar cell polarity pathway involved in axis elongation
protein phosphorylation
Wnt signaling pathway, calcium modulating pathway
positive regulation of gene expression
Wnt signaling pathway
neuron differentiation
bone mineralization
negative regulation of cell growth
adrenal gland development
positive regulation of cell migration
negative regulation of cell migration
response to nutrient levels
activation of protein kinase activity
positive regulation of transforming growth factor beta2 production
protein localization to cell surface
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of GTPase activity
cell fate commitment
maintenance of epithelial cell apical/basal polarity
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
paraxial mesoderm formation
notochord morphogenesis
embryonic skeletal system development
artery morphogenesis
positive regulation of stress fiber assembly
palate development
convergent extension involved in axis elongation
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
cloacal septation
ventricular septum morphogenesis
lung-associated mesenchyme development
negative regulation of cell death
ureteric bud morphogenesis
planar cell polarity pathway involved in gastrula mediolateral intercalation
negative regulation of cartilage development
somite development
neuroendocrine cell differentiation
bicellular tight junction assembly
cellular response to retinoic acid
mesonephric duct development
negative regulation of mesenchymal cell proliferation
positive regulation of protein kinase C signaling
negative regulation of canonical Wnt signaling pathway
negative regulation of fibroblast growth factor production
kidney development
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
non-canonical Wnt signaling pathway
positive regulation of protein kinase activity
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