Invitrogen

WNT3A Polyclonal Antibody, PeproTech®

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

500-P251-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Wnt-3a

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930067

Product Specific Information

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-3a. Anti-Human Wnt-3a-specific antibody was purified by affinity chromatography employing an immobilized Human Wnt-3a matrix.

Sandwich ELISA: To detect Human Wnt-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Wnt-3a (500-P251Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Wnt-3a.

Western Blot: To detect Human Wnt-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Wnt-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; wingless-type MMTV integration site family member 3A; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein

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Gene Aliases: WNT3A

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UniProt ID: (Human) P56704

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Entrez Gene ID: (Human) 89780

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Function(s)
transcription coactivator activity receptor binding frizzled binding frizzled-2 binding protein binding protein domain specific binding co-receptor binding receptor agonist activity intercellular signal molecule
Process(es)
osteoblast differentiation in utero embryonic development positive regulation of cytokine production positive regulation of protein phosphorylation heart looping positive regulation of receptor internalization negative regulation of heart induction by canonical Wnt signaling pathway axon guidance synapse assembly positive regulation of cell proliferation COP9 signalosome assembly negative regulation of neuron projection development Wnt signaling pathway spinal cord association neuron differentiation hippocampus development cell proliferation in forebrain Wnt signaling pathway involved in forebrain neuroblast division dorsal/ventral neural tube patterning hemopoiesis neuron differentiation extracellular matrix organization mammary gland development positive regulation of B cell proliferation positive regulation of protein binding positive regulation of peptidyl-serine phosphorylation cell proliferation in midbrain cellular protein localization positive regulation of catenin import into nucleus skeletal muscle cell differentiation post-anal tail morphogenesis synaptic vesicle recycling inner ear morphogenesis positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of fat cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of mesodermal cell fate specification paraxial mesodermal cell fate commitment positive regulation of skeletal muscle tissue development positive regulation of collateral sprouting in absence of injury negative regulation of axon extension involved in axon guidance negative regulation of neurogenesis positive regulation of sequence-specific DNA binding transcription factor activity palate development canonical Wnt signaling pathway positive regulation of protein tyrosine kinase activity positive regulation of dermatome development canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment regulation of microtubule cytoskeleton organization platelet aggregation cellular response to retinoic acid axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway positive regulation of protein localization to plasma membrane negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex disassembly Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation positive regulation of neural precursor cell proliferation positive regulation of hepatocyte proliferation positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation
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