Proteintech

WT1 Polyclonal Antibody

WT1 Antibody in Immunocytochemistry (ICC/IF)

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WT1 Antibody (12609-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (Product # 12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

WT1 Antibody in Immunoprecipitation (IP)

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Product Details

12609-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:200

View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

1:1,000

ChIP assay (ChIP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WT1 Fusion Protein Ag3337 (1-302 aa encoded by BC032861)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEKGYSTVT FDGTPSYGHT PSHHAAQFPN HSFKHEDPMG QQGSLGEQQY SVPPPVYGCH TPTDSCTGSQ ALLLRTPYSS DNLYQMTSQL ECMTWNQMNL GATLKGVAAG SSSSVKWTEG QSNHSTGYES DNHTTPILCG AQYRMHTHGV FRGIQDVRRV PGVAPTLVRS ASETSEKRPF MCAYPGCNKR YFKLSHLQMH SRKHTGEKPY QCDFKDCERR FSRSDQLKRH QRRHTGVKPF QCKTCQRKFS RSDHLKTHTR THTGEKPFSC RWPSCQKKFA RSDELVRHHN MHQRNMTKLQ LAL (1-302 aa encoded by BC032861)

Target Information

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zearalenone induces oxidative stress and autophagy in goat Sertoli cells.

Ecotoxicology and environmental safety

Liu X,Xi H,Han S,Zhang H,Hu J

Wed Mar 01 00:00:00 EST 2023

Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes.

Pflugers Archiv : European journal of physiology

Vandorpe DH,Heneghan JF,Waitzman JS,McCarthy GM,Blasio A,Magraner JM,Donovan OG,Schaller LB,Shah SS,Subramanian B,Riella CV,Friedman DJ,Pollak MR,Alper SL

Wed Mar 01 00:00:00 EST 2023

WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury.

Theranostics

Hong X,Nie H,Deng J,Liang S,Chen L,Li J,Gong S,Wang G,Zuo W,Hou F,Zhang F

Mon Mar 20 00:00:00 EDT 2023

Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells.

Ecotoxicology and environmental safety

Han S,Zhang H,Liu X,Wen F,Li B,Bie Z,Qiu H,Hu J

Sat Jul 01 00:00:00 EDT 2023

FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway.

Theriogenology

Xi H,Hu Z,Han S,Liu X,Wang L,Hu J

Sun Jan 01 00:00:00 EST 2023

Paeoniflorin binds to VEGFR2 to restore autophagy and inhibit apoptosis for podocyte protection in diabetic kidney disease through PI3K-AKT signaling pathway.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang X,Jiang L,Liu XQ,Huang YB,Wang AL,Zeng HX,Gao L,Zhu QJ,Xia LL,Wu YG

Tue Nov 01 00:00:00 EDT 2022

Paeoniflorin binds to VEGFR2 to restore autophagy and inhibit apoptosis for podocyte protection in diabetic kidney disease through PI3K-AKT signaling pathway.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang X,Jiang L,Liu XQ,Huang YB,Wang AL,Zeng HX,Gao L,Zhu QJ,Xia LL,Wu YG

Tue Nov 01 00:00:00 EDT 2022

Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models.

Nature communications

Song K,Yang X,An G,Xia X,Zhao J,Xu X,Wan C,Liu T,Zheng Y,Ren S,Wang M,Chang G,Cronin SJF,Penninger JM,Jing T,Ou X,Rao S,Liu Z,Zhao XY

Mon Nov 28 00:00:00 EST 2022

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Oncogene

Kwon J,Choi H,Ware AD,Morillo BC,Wang H,Bouker KB,Lu X,Waldman T,Han C

Tue Mar 01 00:00:00 EST 2022

FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells.

Molecular and cellular endocrinology

Xi H,Ren F,Li Y,Xian M,Wang L,Hu J

Sat Jan 15 00:00:00 EST 2022

Restoration of WT1/miR-769-5p axis by HDAC1 inhibition promotes MMT reversal in mesenchymal-like mesothelial cells.

Cell death & disease

Bontempi G,Terri M,Garbo S,Montaldo C,Mariotti D,Bordoni V,Valente S,Zwergel C,Mai A,Marchetti A,Domenici A,Menè P,Battistelli C,Tripodi M,Strippoli R

Thu Nov 17 00:00:00 EST 2022

Restoration of WT1/miR-769-5p axis by HDAC1 inhibition promotes MMT reversal in mesenchymal-like mesothelial cells.

Cell death & disease

Bontempi G,Terri M,Garbo S,Montaldo C,Mariotti D,Bordoni V,Valente S,Zwergel C,Mai A,Marchetti A,Domenici A,Menè P,Battistelli C,Tripodi M,Strippoli R

Thu Nov 17 00:00:00 EST 2022

WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.

Gene

Tang S,Li X,Wu X,Gong Y

Sun Feb 20 00:00:00 EST 2022

Paeoniflorin directly binds to TNFR1 to regulate podocyte necroptosis in diabetic kidney disease.

Frontiers in pharmacology

Wang X,Liu XQ,Jiang L,Huang YB,Zeng HX,Zhu QJ,Qi XM,Wu YG

Sat Sep 24 00:00:00 EDT 2022

Paeoniflorin directly binds to TNFR1 to regulate podocyte necroptosis in diabetic kidney disease.

Frontiers in pharmacology

Wang X,Liu XQ,Jiang L,Huang YB,Zeng HX,Zhu QJ,Qi XM,Wu YG

Sat Sep 24 00:00:00 EDT 2022

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Molecular cell

Heider M,Eichner R,Stroh J,Morath V,Kuisl A,Zecha J,Lawatscheck J,Baek K,Garz AK,Rudelius M,Deuschle FC,Keller U,Lemeer S,Verbeek M,Götze KS,Skerra A,Weber WA,Buchner J,Schulman BA,Kuster B,Fernández-Sáiz V,Bassermann F

Thu Mar 18 00:00:00 EDT 2021

Genome profiling of mismatch repair genes in eight types of tumors.

Cell cycle (Georgetown, Tex.)

Wang S,Guan G,Zou C,Guo Q,Cheng W,Shen S,Dong F,Wu A,Li G,Zhu C

Tue Jun 01 00:00:00 EDT 2021

Genome profiling of mismatch repair genes in eight types of tumors.

Cell cycle (Georgetown, Tex.)

Wang S,Guan G,Zou C,Guo Q,Cheng W,Shen S,Dong F,Wu A,Li G,Zhu C

Tue Jun 01 00:00:00 EDT 2021

Pleural Mesothelial Cells Modulate the Inflammatory/Profibrotic Response During SARS-CoV-2 Infection.

Frontiers in molecular biosciences

Matusali G,Trionfetti F,Bordoni V,Nardacci R,Falasca L,Colombo D,Terri M,Montaldo C,Castilletti C,Mariotti D,Del Nonno F,Capobianchi MR,Agrati C,Tripodi M,Strippoli R

Wed Dec 15 00:00:00 EST 2021

Changes in histology, protein expression, and autophagy in dairy goat testes during nonbreeding season†.

Biology of reproduction

Xi H,Ren F,Li Y,Du Y,Wang L,Hu J

Mon Nov 15 00:00:00 EST 2021

Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.

Molecular psychiatry

Ji F,Wang W,Feng C,Gao F,Jiao J

Sun Aug 01 00:00:00 EDT 2021

Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.

Molecular psychiatry

Ji F,Wang W,Feng C,Gao F,Jiao J

Sun Aug 01 00:00:00 EDT 2021

Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.

Molecular medicine reports

Zhu Z,Liu Q,Sun J,Bao Z,Wang W

Mon Nov 01 00:00:00 EDT 2021

Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.

STAR protocols

Bejoy J,Qian ES,Woodard LE

Fri Dec 17 00:00:00 EST 2021

Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.

EMBO molecular medicine

Kasam RK,Ghandikota S,Soundararajan D,Reddy GB,Huang SK,Jegga AG,Madala SK

Mon Sep 07 00:00:00 EDT 2020

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

eLife

Liang J,Wang N,He J,Du J,Guo Y,Li L,Wu W,Yao C,Li Z,Kee K

Mon Nov 11 00:00:00 EST 2019

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Cell death & disease

Xie K,Xu C,Zhang M,Wang M,Min L,Qian C,Wang Q,Ni Z,Mou S,Dai H,Pang H,Gu L

Wed Dec 04 00:00:00 EST 2019

Analysis of Circulating Tumor Cells in Ovarian Cancer and Their Clinical Value as a Biomarker.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhang X,Li H,Yu X,Li S,Lei Z,Li C,Zhang Q,Han Q,Li Y,Zhang K,Wang Y,Liu C,Mao Y,Wang X,Irwin DM,Guo H,Niu G,Tan H

Mon Sep 10 00:00:00 EDT 2018

Analysis of Circulating Tumor Cells in Ovarian Cancer and Their Clinical Value as a Biomarker.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhang X,Li H,Yu X,Li S,Lei Z,Li C,Zhang Q,Han Q,Li Y,Zhang K,Wang Y,Liu C,Mao Y,Wang X,Irwin DM,Guo H,Niu G,Tan H

Mon Sep 10 00:00:00 EDT 2018

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

JCI insight

Sontake V,Kasam RK,Sinner D,Korfhagen TR,Reddy GB,White ES,Jegga AG,Madala SK

Thu Aug 23 00:00:00 EDT 2018

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

JCI insight

Sontake V,Kasam RK,Sinner D,Korfhagen TR,Reddy GB,White ES,Jegga AG,Madala SK

Thu Aug 23 00:00:00 EDT 2018

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

JCI insight

Sontake V,Kasam RK,Sinner D,Korfhagen TR,Reddy GB,White ES,Jegga AG,Madala SK

Thu Aug 23 00:00:00 EDT 2018

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

JCI insight

Sontake V,Kasam RK,Sinner D,Korfhagen TR,Reddy GB,White ES,Jegga AG,Madala SK

Thu Aug 23 00:00:00 EDT 2018

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Oncogene

Hua G,Lv X,He C,Remmenga SW,Rodabough KJ,Dong J,Yang L,Lele SM,Yang P,Zhou J,Karst A,Drapkin RI,Davis JS,Wang C

Thu Apr 28 00:00:00 EDT 2016

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Oncogene

Hua G,Lv X,He C,Remmenga SW,Rodabough KJ,Dong J,Yang L,Lele SM,Yang P,Zhou J,Karst A,Drapkin RI,Davis JS,Wang C

Thu Apr 28 00:00:00 EDT 2016

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Oncogene

Hua G,Lv X,He C,Remmenga SW,Rodabough KJ,Dong J,Yang L,Lele SM,Yang P,Zhou J,Karst A,Drapkin RI,Davis JS,Wang C

Thu Apr 28 00:00:00 EDT 2016

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Oncogene

Hua G,Lv X,He C,Remmenga SW,Rodabough KJ,Dong J,Yang L,Lele SM,Yang P,Zhou J,Karst A,Drapkin RI,Davis JS,Wang C

Thu Apr 28 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WT; WT33

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Gene Aliases: AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; Wt-1; WT1; WT33

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UniProt ID: (Human) P19544, (Rat) P49952

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Entrez Gene ID: (Human) 7490, (Mouse) 22431, (Rat) 24883

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Function(s)

transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding C2H2 zinc finger domain binding nucleic acid binding DNA binding double-stranded DNA binding metal ion binding C2H2 zinc finger transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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WT1 Polyclonal Antibody

WT1 Antibody (12609-1-AP) in ICC/IF

Product Details

12609-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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