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Invitrogen
WT1 Recombinant Rabbit Monoclonal Antibody (SC06-41)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
WT1 Antibody (MA5-32215) in ICC/IF
Immunofluorescence analysis of Lysozyme was performed using 70% confluent log phase OVCAR-3 and SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with WT1 Recombinant Rabbit Monoclonal Antibody (SC06-41) (Product # MA5-32215) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Ant... View More
Product Details
MA5-32215
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SC06-41
Immunogen
Recombinant protein within Human WT1 aa 59-269
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809502
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WT; WT33
Gene Aliases: AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; Wt-1; WT1; WT33
UniProt ID: (Human) P19544
Entrez Gene ID: (Human) 7490, (Mouse) 22431
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger domain binding
nucleic acid binding
DNA binding
double-stranded DNA binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
regulation of organ formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
germ cell development
thorax and anterior abdomen determination
heart development
sex determination
negative regulation of cell proliferation
RNA splicing
gonad development
male gonad development
tissue development
negative regulation of cell growth
adrenal gland development
male genitalia development
epithelial cell differentiation
glomerulus development
glomerular basement membrane development
adrenal cortex formation
camera-type eye development
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mesenchymal to epithelial transition
positive regulation of heart growth
diaphragm development
cardiac muscle cell fate commitment
visceral serous pericardium development
cellular response to cAMP
cellular response to gonadotropin stimulus
metanephric mesenchyme development
glomerular visceral epithelial cell differentiation
posterior mesonephric tubule development
metanephric epithelium development
metanephric S-shaped body morphogenesis
negative regulation of metanephric glomerular mesangial cell proliferation
positive regulation of male gonad development
negative regulation of female gonad development
positive regulation of metanephric ureteric bud development
metanephros development
mesonephros development
transcription, DNA-templated
apoptotic process
spermatogenesis
single fertilization
regulation of gene expression
negative regulation of translation
sperm motility
positive regulation of apoptotic process
Sertoli cell development
coronary vasculature development
seminiferous tubule development
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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