Proteintech

WTAP Polyclonal Antibody

FIGURE: 1 / 10

WTAP Antibody (10200-1-AP) in WB

HepG2 cells were subjected to SDS PAGE followed by western blot with (Product # 10200-1-AP) WTAP antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

WTAP Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10200-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 26 publications 26 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WTAP Fusion Protein Ag0259 (2-150 aa encoded by BC000383)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPD (2-150 aa encoded by BC000383)

Target Information

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficiency of WTAP in islet beta cells results in beta cell failure and diabetes in mice.

Diabetologia

Li X,Yang Y,Li Z,Wang Y,Qiao J,Chen Z

Thu Jun 01 00:00:00 EDT 2023

m⁶ A promotes planarian regeneration.

Cell proliferation

Cui G,Zhou JY,Ge XY,Sun BF,Song GG,Wang X,Wang XZ,Zhang R,Wang HL,Jing Q,Koziol MJ,Zhao YL,Zeng A,Zhang WQ,Han DL,Yang YG,Yang Y

Mon May 01 00:00:00 EDT 2023

Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m⁶A-dependent recognition of Nrf2 mRNA mediated by SND1.

Journal of experimental & clinical cancer research : CR

Zheng J,Zhang Q,Zhao Z,Qiu Y,Zhou Y,Wu Z,Jiang C,Wang X,Jiang X

Fri May 19 00:00:00 EDT 2023

Profile of N6-methyladenosine of Pb-exposed neurons presents epitranscriptomic alterations in PI3K-AKT pathway-associated genes.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ai S,Li D,Gu X,Xu Y,Wang Y,Wang HL,Chen XT

Tue Aug 01 00:00:00 EDT 2023

Overexpression of VIRMA confers vulnerability to breast cancers via the m⁶A-dependent regulation of unfolded protein response.

Cellular and molecular life sciences : CMLS

Lee Q,Song R,Phan DAV,Pinello N,Tieng J,Su A,Halstead JM,Wong ACH,van Geldermalsen M,Lee BS,Rong B,Cook KM,Larance M,Liu R,Lan F,Tiffen JC,Wong JJ

Fri May 19 00:00:00 EDT 2023

ZBTB7C m6A modification incurred by METTL3 aberration promotes osteosarcoma progression.

Translational research : the journal of laboratory and clinical medicine

An X,Wu W,Yang L,Dong J,Liu B,Guo J,Chen J,Guo B,Cao W,Jiang Q

Fri Sep 01 00:00:00 EDT 2023

FTO-dependent m⁶A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke.

Nature communications

Li B,Xi W,Bai Y,Liu X,Zhang Y,Li L,Bian L,Liu C,Tang Y,Shen L,Yang L,Gu X,Xie J,Zhou Z,Wang Y,Yu X,Wang J,Chao J,Han B,Yao H

Mon Jan 30 00:00:00 EST 2023

A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells.

PLoS biology

Porman AM,Roberts JT,Duncan ED,Chrupcala ML,Levine AA,Kennedy MA,Williams MM,Richer JK,Johnson AM

Tue Nov 01 00:00:00 EDT 2022

m⁶A modification confers thermal vulnerability to HPV E7 oncotranscripts via reverse regulation of its reader protein IGF2BP1 upon heat stress.

Cell reports

Wang L,Zhan G,Maimaitiyiming Y,Su Y,Lin S,Liu J,Su K,Lin J,Shen S,He W,Wang F,Chen J,Sun S,Xue Y,Gu J,Chen X,Zhang J,Zhang L,Wang Q,Chang KJ,Chiou SH,Björklund M,Naranmandura H,Cheng X,Hsu CH

Tue Oct 25 00:00:00 EDT 2022

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH).

Nature communications

Li X,Ding K,Li X,Yuan B,Wang Y,Yao Z,Wang S,Huang H,Xu B,Xie L,Deng T,Chen XW,Chen Z

Thu Aug 04 00:00:00 EDT 2022

Dynamic control of chromatin-associated m⁶A methylation regulates nascent RNA synthesis.

Molecular cell

Xu W,He C,Kaye EG,Li J,Mu M,Nelson GM,Dong L,Wang J,Wu F,Shi YG,Adelman K,Lan F,Shi Y,Shen H

Thu Mar 17 00:00:00 EDT 2022

WTAP Targets the METTL3 m⁶A-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection.

Journal of virology

Sacco MT,Bland KM,Horner SM

Wed Nov 23 00:00:00 EST 2022

WTAP Targets the METTL3 m⁶A-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection.

Journal of virology

Sacco MT,Bland KM,Horner SM

Wed Nov 23 00:00:00 EST 2022

The expression of m6A enzymes in the hippocampus of diabetic cognitive impairment mice and the possible improvement of YTHDF1.

Brain research

Li M,Zhong X,Zhao Z,Zeng Z,Yuan Q,Xiao X,Zu X,Liu J

Tue Feb 15 00:00:00 EST 2022

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions.

Nature communications

Li Y,He X,Lu X,Gong Z,Li Q,Zhang L,Yang R,Wu C,Huang J,Ding J,He Y,Liu W,Chen C,Cao B,Zhou D,Shi Y,Chen J,Wang C,Zhang S,Zhang J,Ye J,You H

Wed Oct 26 00:00:00 EDT 2022

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions.

Nature communications

Li Y,He X,Lu X,Gong Z,Li Q,Zhang L,Yang R,Wu C,Huang J,Ding J,He Y,Liu W,Chen C,Cao B,Zhou D,Shi Y,Chen J,Wang C,Zhang S,Zhang J,Ye J,You H

Wed Oct 26 00:00:00 EDT 2022

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions.

Nature communications

Li Y,He X,Lu X,Gong Z,Li Q,Zhang L,Yang R,Wu C,Huang J,Ding J,He Y,Liu W,Chen C,Cao B,Zhou D,Shi Y,Chen J,Wang C,Zhang S,Zhang J,Ye J,You H

Wed Oct 26 00:00:00 EDT 2022

METTL3-mediated m⁶A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Molecular therapy : the journal of the American Society of Gene Therapy

Jiang L,Liu X,Hu X,Gao L,Zeng H,Wang X,Huang Y,Zhu W,Wang J,Wen J,Meng X,Wu Y

Wed Apr 06 00:00:00 EDT 2022

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma.

Discover. Oncology

Kong F,Wang K,Wang L

Fri Nov 25 00:00:00 EST 2022

YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity.

Retrovirology

N'Da Konan S,Ségéral E,Bejjani F,Bendoumou M,Ait Said M,Gallois-Montbrun S,Emiliani S

Mon Jan 31 00:00:00 EST 2022

PGE₂ -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.

The EMBO journal

Tao X,Du R,Guo S,Feng X,Yu T,OuYang Q,Chen Q,Fan X,Wang X,Guo C,Li X,Xue F,Chen S,Tong M,Lazarus M,Zuo S,Yu Y,Shen Y

Tue Aug 16 00:00:00 EDT 2022

Expression pattern of alkB homolog 5 in goat testis and its role in spermatogonial stem cells.

Cell and tissue research

Liu Z,Cai Y,Deng M,Li D,Leng Q,Shi L,Tang Y,Wang F,Wan Y

Sat Jan 01 00:00:00 EST 2022

Vitamin B₁₂ Deficiency Dysregulates m6A mRNA Methylation of Genes Involved in Neurological Functions.

Molecular nutrition & food research

Mosca P,Robert A,Alberto JM,Meyer M,Kundu U,Hergalant S,Umoret R,Coelho D,Guéant JL,Leheup B,Dreumont N

Wed Sep 01 00:00:00 EDT 2021

m⁶A RNA methylation regulates the fate of endogenous retroviruses.

Nature

Chelmicki T,Roger E,Teissandier A,Dura M,Bonneville L,Rucli S,Dossin F,Fouassier C,Lameiras S,Bourc'his D

Mon Mar 01 00:00:00 EST 2021

Integrated analysis of m⁶A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension.

Aging

Zeng Y,Huang T,Zuo W,Wang D,Xie Y,Wang X,Xiao Z,Chen Z,Liu Q,Liu N,Xiao Y

Mon Jul 26 00:00:00 EDT 2021

EGR2-mediated regulation of m⁶A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Cell death & disease

Ying Y,Ma X,Fang J,Chen S,Wang W,Li J,Xie H,Wu J,Xie B,Liu B,Wang X,Zheng X,Xie L

Thu Jul 29 00:00:00 EDT 2021

EGR2-mediated regulation of m⁶A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Cell death & disease

Ying Y,Ma X,Fang J,Chen S,Wang W,Li J,Xie H,Wu J,Xie B,Liu B,Wang X,Zheng X,Xie L

Thu Jul 29 00:00:00 EDT 2021

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Nature

Xu W,Li J,He C,Wen J,Ma H,Rong B,Diao J,Wang L,Wang J,Wu F,Tan L,Shi YG,Shi Y,Shen H

Mon Mar 01 00:00:00 EST 2021

Degradation of WTAP blocks antiviral responses by reducing the m⁶ A levels of IRF3 and IFNAR1 mRNA.

EMBO reports

Ge Y,Ling T,Wang Y,Jia X,Xie X,Chen R,Chen S,Yuan S,Xu A

Thu Nov 04 00:00:00 EDT 2021

Degradation of WTAP blocks antiviral responses by reducing the m⁶ A levels of IRF3 and IFNAR1 mRNA.

EMBO reports

Ge Y,Ling T,Wang Y,Jia X,Xie X,Chen R,Chen S,Yuan S,Xu A

Thu Nov 04 00:00:00 EDT 2021

Reprogramming of m⁶A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia.

RNA biology

Wang YJ,Yang B,Lai Q,Shi JF,Peng JY,Zhang Y,Hu KS,Li YQ,Peng JW,Yang ZZ,Li YT,Pan Y,Koeffler HP,Liao JY,Yin D

Fri Jan 01 00:00:00 EST 2021

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N⁶-methyladenosine methylation of Parp10 mRNA.

Nature cell biology

Gao XQ,Zhang YH,Liu F,Ponnusamy M,Zhao XM,Zhou LY,Zhai M,Liu CY,Li XM,Wang M,Shan C,Shan PP,Wang Y,Dong YH,Qian LL,Yu T,Ju J,Wang T,Wang K,Chen XZ,Wang YH,Zhang J,Li PF,Wang K

Sun Nov 01 00:00:00 EDT 2020

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N⁶-methyladenosine methylation of Parp10 mRNA.

Nature cell biology

Gao XQ,Zhang YH,Liu F,Ponnusamy M,Zhao XM,Zhou LY,Zhai M,Liu CY,Li XM,Wang M,Shan C,Shan PP,Wang Y,Dong YH,Qian LL,Yu T,Ju J,Wang T,Wang K,Chen XZ,Wang YH,Zhang J,Li PF,Wang K

Sun Nov 01 00:00:00 EDT 2020

WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1.

Molecular cancer

Chen Y,Peng C,Chen J,Chen D,Yang B,He B,Hu W,Zhang Y,Liu H,Dai L,Xie H,Zhou L,Wu J,Zheng S

Thu Aug 22 00:00:00 EDT 2019

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Cell reports

Moindrot B,Cerase A,Coker H,Masui O,Grijzenhout A,Pintacuda G,Schermelleh L,Nesterova TB,Brockdorff N

Tue Jul 28 00:00:00 EDT 2015

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Cell reports

Moindrot B,Cerase A,Coker H,Masui O,Grijzenhout A,Pintacuda G,Schermelleh L,Nesterova TB,Brockdorff N

Tue Jul 28 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Female-lethal(2)D homolog; hFL(2)D; PNAS-132; Pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein

View more View less

Gene Aliases: 2810408K05Rik; 9430038B09Rik; KIAA0105; Mum2; RGD1563824; WTAP

View more View less

UniProt ID: (Human) Q15007, (Mouse) Q9ER69

View more View less

Entrez Gene ID: (Human) 9589, (Rat) 499020, (Mouse) 60532

View more View less

Function(s)

protein binding

Process(es)

regulation of alternative mRNA splicing, via spliceosome mRNA methylation mRNA processing cell cycle RNA splicing gene expression multicellular organism development regulation of mRNA splicing, via spliceosome

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

WTAP Polyclonal Antibody

WTAP Antibody (10200-1-AP) in WB

Product Details

10200-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!