Proteintech

WTAP Monoclonal Antibody (4A10G9)

WTAP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

WTAP Antibody (60188-1-IG) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 60188-1-IG (WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

WTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

60188-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 45 publications 45 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 3 publications 3 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4A10G9

Immunogen

WTAP Fusion Protein Ag0259 (2-150 aa encoded by BC000383)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPD (2-150 aa encoded by BC000383)

Target Information

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer.

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WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization.

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WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization.

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WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization.

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WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization.

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Human genomics

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Human genomics

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Exon junction complex shapes the m⁶A epitranscriptome.

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WTAP promotes oesophageal squamous cell carcinoma development by decreasing CPSF4 expression in an m⁶A-dependent manner.

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WTAP promotes oesophageal squamous cell carcinoma development by decreasing CPSF4 expression in an m⁶A-dependent manner.

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PDGF signaling inhibits mitophagy in glioblastoma stem cells through N⁶-methyladenosine.

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METTL14 Regulates Intestine Cellular Senescence through m⁶A Modification of Lamin B Receptor.

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PGE₂ -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.

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miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.

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miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.

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Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m⁶A Methylation.

Molecular cell

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Thu Nov 19 00:00:00 EST 2020

Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m⁶A Methylation.

Molecular cell

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Thu Nov 19 00:00:00 EST 2020

Validation strategies for antibodies targeting modified ribonucleotides.

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Alteration of N⁶-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.

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AMPK facilitates intestinal long-chain fatty acid uptake by manipulating CD36 expression and translocation.

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Wed Apr 01 00:00:00 EDT 2020

Direct RNA sequencing reveals m⁶A modifications on adenovirus RNA are necessary for efficient splicing.

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Thu Nov 26 00:00:00 EST 2020

Direct RNA sequencing reveals m⁶A modifications on adenovirus RNA are necessary for efficient splicing.

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Thu Nov 26 00:00:00 EST 2020

Identification of WTAP-related genes by weighted gene co-expression network analysis in ovarian cancer.

Journal of ovarian research

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Wed Sep 30 00:00:00 EDT 2020

ANKLE1 N⁶ -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability.

International journal of cancer

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Mon Jun 15 00:00:00 EDT 2020

RNA m⁶ A modification enzymes shape innate responses to DNA by regulating interferon β.

Genes & development

Rubio RM,Depledge DP,Bianco C,Thompson L,Mohr I

Sat Dec 01 00:00:00 EST 2018

RNA m⁶ A modification enzymes shape innate responses to DNA by regulating interferon β.

Genes & development

Rubio RM,Depledge DP,Bianco C,Thompson L,Mohr I

Sat Dec 01 00:00:00 EST 2018

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m⁶A machinery component Wtap/Fl(2)d.

Genes & development

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Thu Mar 01 00:00:00 EST 2018

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m⁶A machinery component Wtap/Fl(2)d.

Genes & development

Knuckles P,Lence T,Haussmann IU,Jacob D,Kreim N,Carl SH,Masiello I,Hares T,Villaseñor R,Hess D,Andrade-Navarro MA,Biggiogera M,Helm M,Soller M,Bühler M,Roignant JY

Thu Mar 01 00:00:00 EST 2018

METTL3 regulates WTAP protein homeostasis.

Cell death & disease

Sorci M,Ianniello Z,Cruciani S,Larivera S,Ginistrelli LC,Capuano E,Marchioni M,Fazi F,Fatica A

Mon Jul 23 00:00:00 EDT 2018

METTL3 regulates WTAP protein homeostasis.

Cell death & disease

Sorci M,Ianniello Z,Cruciani S,Larivera S,Ginistrelli LC,Capuano E,Marchioni M,Fazi F,Fatica A

Mon Jul 23 00:00:00 EDT 2018

Synaptic N⁶-methyladenosine (m⁶A) epitranscriptome reveals functional partitioning of localized transcripts.

Nature neuroscience

Merkurjev D,Hong WT,Iida K,Oomoto I,Goldie BJ,Yamaguti H,Ohara T,Kawaguchi SY,Hirano T,Martin KC,Pellegrini M,Wang DO

Sun Jul 01 00:00:00 EDT 2018

Synaptic N⁶-methyladenosine (m⁶A) epitranscriptome reveals functional partitioning of localized transcripts.

Nature neuroscience

Merkurjev D,Hong WT,Iida K,Oomoto I,Goldie BJ,Yamaguti H,Ohara T,Kawaguchi SY,Hirano T,Martin KC,Pellegrini M,Wang DO

Sun Jul 01 00:00:00 EDT 2018

Region-specific RNA m⁶A methylation represents a new layer of control in the gene regulatory network in the mouse brain.

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Fri Sep 01 00:00:00 EDT 2017

Region-specific RNA m⁶A methylation represents a new layer of control in the gene regulatory network in the mouse brain.

Open biology

Chang M,Lv H,Zhang W,Ma C,He X,Zhao S,Zhang ZW,Zeng YX,Song S,Niu Y,Tong WM

Fri Sep 01 00:00:00 EDT 2017

Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.

Oncotarget

Xi Z,Wang P,Xue Y,Shang C,Liu X,Ma J,Li Z,Li Z,Bao M,Liu Y

Tue Apr 11 00:00:00 EDT 2017

Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.

Oncotarget

Xi Z,Wang P,Xue Y,Shang C,Liu X,Ma J,Li Z,Li Z,Bao M,Liu Y

Tue Apr 11 00:00:00 EDT 2017

Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.

Oncotarget

Xi Z,Wang P,Xue Y,Shang C,Liu X,Ma J,Li Z,Li Z,Bao M,Liu Y

Tue Apr 11 00:00:00 EDT 2017

Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.

Oncotarget

Xi Z,Wang P,Xue Y,Shang C,Liu X,Ma J,Li Z,Li Z,Bao M,Liu Y

Tue Apr 11 00:00:00 EDT 2017

The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.

Molecular cell

Lin S,Choe J,Du P,Triboulet R,Gregory RI

Thu May 05 00:00:00 EDT 2016

The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.

Molecular cell

Lin S,Choe J,Du P,Triboulet R,Gregory RI

Thu May 05 00:00:00 EDT 2016

WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.

Journal of molecular neuroscience : MN

Xi Z,Xue Y,Zheng J,Liu X,Ma J,Liu Y

Sat Oct 01 00:00:00 EDT 2016

WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.

Journal of molecular neuroscience : MN

Xi Z,Xue Y,Zheng J,Liu X,Ma J,Liu Y

Sat Oct 01 00:00:00 EDT 2016

WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.

Journal of molecular neuroscience : MN

Xi Z,Xue Y,Zheng J,Liu X,Ma J,Liu Y

Sat Oct 01 00:00:00 EDT 2016

WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.

Journal of molecular neuroscience : MN

Xi Z,Xue Y,Zheng J,Liu X,Ma J,Liu Y

Sat Oct 01 00:00:00 EDT 2016

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Science (New York, N.Y.)

Geula S,Moshitch-Moshkovitz S,Dominissini D,Mansour AA,Kol N,Salmon-Divon M,Hershkovitz V,Peer E,Mor N,Manor YS,Ben-Haim MS,Eyal E,Yunger S,Pinto Y,Jaitin DA,Viukov S,Rais Y,Krupalnik V,Chomsky E,Zerbib M,Maza I,Rechavi Y,Massarwa R,Hanna S,Amit I,Levanon EY,Amariglio N,Stern-Ginossar N,Novershtern N,Rechavi G,Hanna JH

Fri Feb 27 00:00:00 EST 2015

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Science (New York, N.Y.)

Fri Feb 27 00:00:00 EST 2015

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Female-lethal(2)D homolog; hFL(2)D; PNAS-132; Pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein

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Gene Aliases: 2810408K05Rik; 9430038B09Rik; KIAA0105; Mum2; RGD1563824; WTAP

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UniProt ID: (Human) Q15007, (Mouse) Q9ER69

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Entrez Gene ID: (Human) 9589, (Rat) 499020, (Mouse) 60532

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Function(s)

protein binding

Process(es)

regulation of alternative mRNA splicing, via spliceosome mRNA methylation mRNA processing cell cycle RNA splicing gene expression multicellular organism development regulation of mRNA splicing, via spliceosome

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WTAP Monoclonal Antibody (4A10G9)

WTAP Antibody (60188-1-IG) in ICC/IF

Product Details

60188-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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