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WWC1 Antibody (CF801568) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WWC1 (RC214592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-WWC1. Positive lysates LY414708 (100 µg) and LC414708 (20 µg) can be purchased separately from OriGene.
Product Details
CF801568
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI1H8
Immunogen
Full length human recombinant protein of human WWC1 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
WWC1 (Protein KIBRA) is a probable regulator of the Hippo/SWH signaling pathway. This pathway plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. WWC1, along with NF2, can synergistically induce the phosphorylation of LATS1 and LATS2. It acts as a transcriptional coactivator of ESR1 - which plays an essential role in DYNLL1-mediated ESR1 transactivation. WWC1 also functions as a regulatior of collagen-stimulated activation of the ERK/MAPK cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10865; FLJ23369; HBeAg-binding protein 3; KIBRA; Kidney and brain protein; Protein KIBRA; protein phosphatase 1, regulatory subunit 168; protein WWC1; WW domain-containing protein 1; WW, C2 and coiled-coil domain containing 1
Gene Aliases: HBEBP3; HBEBP36; KIAA0869; KIBRA; MEMRYQTL; PPP1R168; WWC1
UniProt ID: (Human) Q8IX03
Entrez Gene ID: (Human) 23286
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell migration
establishment of cell polarity
regulation of intracellular transport
hippo signaling
regulation of hippo signaling
negative regulation of hippo signaling
positive regulation of MAPK cascade
negative regulation of organ growth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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